The Protective Effect Of Tangshenfang On Early Diabetic Nephropathy In Rats

Objective:Through the pharmacodynamics study to research the protective effect of Yishenhuoxuefang on rats with Ⅰ early diabetic nephropathy and discuss its possible protective mechanism.Metheds:110 healthy male Wistar rats. After one week adaption, 18 rats were randomly selected as the normal contron group. The rest rats were used for establishing model.Before establishing model, forbidding food but drinking freely, the rats were given intraperitoneal injection of STZ(55mg/kg), the normal group were injected with the equal volume of 1% citric acid-sodium at the same conditions. After 72 hours, we measured the blood glucose and considered the blood glucose more than 13.8mmol/L as the successful models.The rats were randomly divided into six groups, according to the following dose by gavage once everyday in 8 weeks, the high dose of Tangshenfang 2g/kg, the medium dose of Tangshenfang 1g/kg, the small dose of Tangshenfang 0.5g/kg, the positive drug(Benazepril) group 1mg/kg. At the end of the eighth weeks, collectting 24 hours urine of rats for the urinary albumin excretion rate(UAER). And all rats were anesthetized, we got the blood from abdominal artery for biochemical test, and collected the kidney and the pancreas to calculate KW/BW and observe morphological and detect the expression of smad7 and nephrin.Results:1.Model group food and water intake, urine output significantly increased, body weight decreased; Compared with model group, Tangshenfang ofthe high, medium group and Benazepril group, all of above symptoms have improved.2.Compared with normal group, blood glucose of model group rats were significantly higher(P<0.01),INS content of model group were significantly lower(P<0.01); Compared with model group, Tangshenfang ofthe high, medium group and Benazepril groupcan significantly lower blood glucose levels(P<0.01), and can significantly increase INS level(P<0.01).3. Compared with normal group, serum CHO level significantly increased(P<0.01);Compared with model group, Tangshenfang ofthe high, medium group and Benazepril group can reduce CHO level(P<0.05).4. Compared with normal group, serum BUN of model group were significantly increase(P<0.01); Compared with model group, Tangshenfang ofthe high, medium group and Benazepril group can significantly decrease BUN level(P<0.05).5. Compared with normal group, urine m ALB and UAER content of model group were significantly increased(P<0.01), Compared with model group, Tangshenfang ofthe high, medium group and Benazepril group can significantly reduce UREA and INS level(P<0.01).6. Compared with normal group, serum T-AOC and GSH-PX content of model group were significantly lower(P<0.01); Compared with model group, Tangshenfang ofthe high,medium group and Benazepril group can significantly increase T-AOC and GSH-PX level(P<0.05).7. Compared with normal group, KW/BW of model group were significantly increased(P<0.01);Compared with model group, Tangshenfang ofthe high, medium group and Benazepril group can significantly reduce KW/BW,inhibit renal hypertrophy.8. Compared with normal group, the renal tissue of model group were seen significant pathological damage; Compared with model group,Tangshenfang ofthe high, medium group and Benazepril groupcan reduce renal pathology damage.9.Compared with normal group, the expression of Smad7 and Nephrin protein content of model group significantly lower(P<0.01); Compared with model group, Tangshenfang ofthe high, medium group and Benazepril groupcan significantly increase Smad7 expression and Nephrin protein content(P<0.01).Conclusion:Tangshenfang can improve the general symptoms of DN obviously, and improve glucose and lipid metabolism, and have strong antioxidant ability, and reduce kidney hypertrophy index and pathological damage, and regulate the expression of Smad7 and alleviate glomerular podocyte damage.