Intervention Mechanism Of Shenmai Injection On Cardiomyocyte Injury In Post Cardiopulmonary Resuscitation Rabbits

Objective:To observe the effect of Shenmai Injection on restoration of spontaneous criculation(ROSC) and ATPase(Na+-K+atpase and Ca2+-Mg2+atpase) activity of cardiomyocyte in rabbits of cardiac arrest after cardiopulmonary resuscitation(CPR).To explore the intervention mechanism of Shenmai Injection on cardiomyocyte injury after CPR. Methods:Total of 49 rabbits were randomly divided into 7 groups of each 7:the sham group, the normal saline group(24h group,48 h group,72 h group), and Shenmai group(24h group,48 h group,72 h group).Rabbit cardiac arrest model was made with asphyxia by clamping the traches of rabbit.Rabbits in Shenmai group were injected with 2ml/kg Shenmai Injection immediately at the beginning of CPR, and the same dose of Shenmai Injection 10 minutes after CPR. Rabbits in the saline group were injected with the same volume of physiological saline instead at the same intervals as previous group.Recorded the time of cardiac arrest and ROSC of the two groups.The level of blood lactate and creatine kinase isoenzyme(CK-MB)were measured in all rabbits before asphyxia and different time after ROSC. Cardiomyocyte ATPase(Na+-K+atpase and Ca2+-Mg2+atpase) activities were mesured by using the colorimetric method.Compared dynamic levels of blood lactate and serum CK-MB and cardiomyocyte ATPhase activities between the two groups after CPR. Observed ultrastructure damage changes of cardiomyocyte under the elecron microscope. Results:1.There was no statestically significant difference in the time of cardiac arrest of rabbits between the two gruops, but the ROSC emerged sooner in Shenmai group(p<0.05).2.There was no statistically significant difference in the levels of blood lactate between the normal group and Shenmai group before asphyxia and 2, 4 hours after CPR,but there was a lower level in Shenmai group 6 hours after CPR(p<0.05).3.There was no statistically significant difference in the levels of serum CK-MB between the normal group and Shenmai group before asphyxia and 6 hours after CPR.There was a lower level in Shenmai group 12, 24, 48, 72 hours after CPR(p<0.05).4.Cardiomyocyte ATPase activities significantly reduced after CPR, and radually rose 24,48,72 hours after CPR. Cardiomyocyte ATPase activities of Shenmai group were always higher(p<0.05).5.Under the electron microscopy,cardiomyocyte ultrastructure of Shenmai group was mild damaged compared. Conclusion:1.Shenmai Injection can shorten ROSC time of cardiac arrest in rabbits.2.Shenmai Injecton can reduce the level of blood lactate and serum CK-MB after CPR, and it can improve cardiomyocyte ATPatpase activities and protect the structural integrity of cardiomyocyte, alleviate cardiomyocyte injury after CPR.