The Study Of Histology And Acetylcholinesterase Of Rabbit Trigeminal Ganglion After Percutaneous Microballoon Compression

Objectives Trigeminal neuralgia is a kind of functional neurosurgical disease. Percutaneous balloon compression (PBC) of trigeminal neuralgia is a new percutaneous minimally invasive surgical approach. The surgeons puncture through Hartel anterior approach under the guidance of X-rays, slip the Fogarty balloon catheter, inject the non-ionic contrast agent Omnipaque, fill the balloon into pear-shaped and pressure Meckel’s chamber. The advantages of PBC are high cure rate, simple operation, shorter operative time, little pain and safe. Some scholars found that rabbit trigeminal nerve fibers appeared axonal injury and demyelination after PBC by some basic researches. What’s more, some other researchers believe that acetylcholinesterase plays a certain role in nerve cell proliferation, differentiation and growth. The research of acetylcholinesterase lay the theoretical foundation in clinical research nerves, motor system injury and repair. There is no study reporting the dynamic changes of histological and acetylcholinesterase in trigeminal nerve after PBC. This research aims to study these changes and explore the principles and mechanisms in the treatment of trigeminal neuralgia by PBC.Methods1. Animal model establishment of percutaneous microballoon compression.2.H&E observation of trigeminal ganglion tissue and diameter measurement of trigeminal ganglion nerve cells.3.AChE measurement of trigeminal ganglion tissue by immunohistochemistry.Results1. Animal model of trigeminal ganglion injury was established by percutaneous microballoon compression.2.After PBC, the large diameter myelinated nerve appeared axon swelled, disrupts and demyelinated. Axonal swelling and fragmentation was exhibited uniformly. The diameters of nerve cells in normal control group and 1 day after PBC group are dramatically longer than other three groups(p<0.05).3.The AChE gray value of the 7 days after PBC group is statistically significant higher than other four groups(p<0.05).Conclusions1.After PBC, the trigeminal ganglion appeared sequential histopathological changes such as demyelinated, mild swelling and analosis, which reminds us that these changes are related to the treatment of trigeminal neuralgia.2.The content of AChE in trigeminal ganglion tissue reduces after PBC, reaching its minimum in the 7th day after operation, which reminds that AChE plays a certain role in nerve injury and repair.