| Objective Alcoholic liver fibrosis (AHF) is in alcohol and its metabolic product of chronic persistent liver damage, liver tissue extracellular matrix (ECM) excessive proliferation and the result of abnormal deposition. AHF is an early stage of alcoholic cirrhosis of the liver, alcoholic hepatitis, alcoholic liver cirrhosis development for the middle stage and the only way. More often the incidence of alcoholic liver disease in western countries, but as people lifestyle change in recent years, there is an upward trend in the incidence of the disease in China. AHF is a stage in the development of alcoholic liver disease, mild could be reversed, but when irreversible, it into the probability of cirrhosis of the liver. Therefore explore the mechanism of alcoholic liver fibrosis, in control and even reverse early liver fibrosis has important clinical significance. It also reflects the traditional Chinese medicine "Hyman both" thinking of diagnosis and treatment. Now it is still lack of specific clinical liver fibrosis drug resistance, To the overall adjustment of Chinese medicine as a starting point, treatment based on syndrome differentiation as its diagnostic and treatment, has a unique curative effect in treatment of liver fibrosis. Thanh Hoa (Qinghua Yudu Fang) is my professor for many years experience in the clinical treatment of alcoholic liver fibrosis, with clear the blood poison, feminine liver function.. The project intention of Th17/Treg balance role in alcoholic liver fiber mechanism, ways and influencing factors of in-depth study, from the perspective of cellular immunology to explore Chinese medicine Thanh Hoa in the resistance to the curative effect of alcoholic liver fibrosis.Methods 60 clean set of Wistar rats, Weight 200 soil 10 g, male, Groups of normal feeding after 1 week, After weighing were randomly divided into 2 groups:10 normal control group,50model group. After building for liver fibrosis formation, randomly assigned module is to be built according to the weight:The model control group 10 only, Compound turtle shell soft liver group 10, Clear the blood poison low dose group of 10, Clear the blood poison dose group of 10, Clear the blood poison with high dose group of 10; With picric acid. Groups such as gastric volume gives corresponding drugs after 4 weeks,2 ml of blood in the heparin anticoagulant centrifuge tube, used for the preparation of peripheral blood mononuclear cell (PBMC), Another 2 ml of blood in anticoagulant tube, using enzyme-linked immunoassay (ELISA) to detect level of IL-17. Take fresh liver tissue, used for electron microscope. Same part and take big hepatic lobe of liver tissue, preparation of mononuclear cells in the liver parenchyma. Using flow cytometry instrument between groups of rats was detected in peripheral blood and the expression of Th17 and Treg cells in the liver tissue, After the original data collection, the Cell can be applied to Quest software system is analyzed.Results1.Clear blood poison fang group and normal control group, model control group, compound turtle shell soft liver piece of group comparison, keep a better balance of Th17/Treg, (P<0.05).2.Qing blood poison side high dose group is lower than pure blood poison side, middle dose group of Th 17/Treg balance to maintain better. (P<0.05)3.The expression of IL-17:Compared with normal control group, model control group, Compound turtle shell soft liver group, Clear the blood poison party group, In the expression of IL-17 are reduced. Difference was statistically significant (P< 0.05). (P<0.05)Conclusion1、"qing blood poison" on alcoholic liver fibrosis has obvious anti fibrosis.2、"Pure blood poison" alcohol resistance mechanism of liver fibrosis and helper T cells (Th17) and the expression of regulatory T cells (Treg).3、"Pure blood poison" resisting alcoholic liver fibrosis associated with control the secretion of IL-17. |
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