The Role Of The Combined Detection Of CIC-C1q, RF, And CCP In The Diagnosis Of Rheumatoid Arthritis And Other Arthritis

BackgroundThe rheumatoid arthritis is a common autoimmune abnormality disease. The cause of the disease RA may related to genetic, infection, estrogen levels and some other factors. But we are not entirely clear about it temporarily. At present, the diagnosis of RA mainly depend on the serology testing index of clinical manifestations, X-ray examination, rheumatoid factor, and anti-cyclic citrullinated peptide antibody, etc. As we known, the autoimmunity reflection takes an important part in the pathological process of RA synovitis. Many kinds of auto-antibodies, citrullinated protein, and immune complexes have been found in the blood and synovia of RA patients. So the relationship between CIC and auto-antibodies, and the level of CIC are remain to a further study. To discuss the effect of CIC in RA and other arthritis, and the inner relationship between CIC and other serology testing indexes, we start the research through the test of CIC-C1q, RF, and CCP.ObjectiveTo discuss the level of CIC-Clq in RA and other arthritis, and study the application of the combined detection of RF and CCP.Method1. Experimental groups:we have RA group, arthritis group and healthy control group. 60 people in each group. The first two groups are patients being treated and diagnosed as for this disease in Luoyang Central Hospital from June,2014 to June,2015. The diagnosis of RA group meets the RA diagnostic classification criteria which ACR and EULAR have revised in 2010. The other diagnosis of arthritis all meets the corresponding diagnostic criteria. The arthritis group are mainly about the disease that are common in clinical which need differential diagnosis of RA. The healthy control group are random sampling health examination population in Luoyang Central Hospital. All Serum specimen that collected from the three groups need keep in -70℃.2. Index detection:We take the routine detection of RF and CCP in three groups. Compare the differences of all the index in three groups and analyze whether it has statistically significant differences. We detect the level of CIC-C1q in three groups through C1q solid phase method, compare the differences of the CIC-C1q level among the three groups and analyze whether it has statistically significant differences.3. Statistical and analysis:Using the software SPSS 19.0 to discuss the relationship among RF, CCP and CIC-C1q. Compare the differences among the three groups. We consider that there exist statistically significant differences when P<0.05.Results1. The 60 patients’ mean value of RF,CCP in RA group are 117.17±11.08, 545.64±93.73, In arthritis group they are 21.42±4.66,13.21±3.32, In healthy control gro-up they are 7.65±0.64,5.07±0.66. We can learn from the non-parametric test by using the software SPSS 19.0 that there are significant differences in the level of RF and CCP between RA group and the other two groups. And there are no significant difference in the level of RF and CCP between arthritis group and healthy control group.(P=0.459 and 0.865, P>0.05).2. The main value of CIC-C1q in the three groups are 136.47±15.25,89.67±13.85, 27.64±2.27. We can learn there are significant differences in the CIC-C1q level among the groups from non-parametric test by using the software SPSS 19.0.3. The detection rate of the combined detection of RF, CCP and CIC-C1q is 98.4%. It improves greatly compared with 83.3% of RF alone detection and 76.7% of CCP alone detection. Through the X2 testing, P<0.05, It prompts that the significant differences exist.ConclusionThe CIC-C1q level among the three groups has significant differences. The combined detection with RF and CCP has greater value in early diagnosis of RA and in the differential diagnosis between RA and other arthritis.