| Objective:To observe the in vitro and in vivo of Adriamycin-N-succinyl chitosan(SCS) sustained release nanometer particles following implantation, and evaluate the local chemotherapeutic effect on rats bearing C6 gliomas.Methods:The matrix with adriamycin was prepared. Gelatin and agar were used as major materials. The releasing effects of Adriamycin-SCS sustained release nanometer particles were determined in artificial cerebrospinal fluid or in 20 SD rats brains by the method of fluorometry. The median survival time (MST) was measured in 20 ICR rats bearing intracranial C6 gliomas, the inhibitory effect of regional administration of adriamycin-SCS on growth of subcutaneous tumors were evaluated in 20 rats.Results:Constant-release of adriamycin-SCS lasted over 30 days in artificial CSF or normal rat brains;Local controlled release of adriamycin could prolong the survival of rats bearing C6 gliomas with the median survival time being 35 days. The rats in experimental group were subcutaneous tumor growth slowly, a statistically significant difference in tumor volume between the two groups (χ2=7.68, P<0.05), apoptosis index of tumor:control group:6.4%, experimental group:59.3%.Statistical Analysis showed a statistically significant difference between the two groups (χ2=23.59, P<0.05).Conclusion:With high regional concentration and avoidance of toxicity of systemic chemotherapy, control released chemotherapy of Adriamycin-SCS sustained nanometer particles remarkably inhibited the growth of C6 gliomas. |
