The Effects Of FTY720 On Retinal Photoreceptor Cells And Microglial Following Light-Induced Degeneration In Rat Retinal

OBLECTIVE To investigate the effects of FTY720 on Retinal photoreceptor cells and microglial following light-induced degeneration in rat retinal. METHODS 120 SD rats were randomly divided into four groups:a FTY720 group, a Solvent control group, a model group,a normal group, each group were 30 rats.The normal group does not give processing, the FTY720 group, Solvent control group and model group establish retinal light injury mode, The model group only accept illumination.FTY720 was injected into abdominal cavity of the rats in FTY720 group 0.5 hours before light exposure..50%DMSO was injected into abdominal cavity of the rats in solvent control. The expressions of microglial in rat retinal were quantified using flow cytometry, the expressions of IL-1βin rat retinal were examined by ELISA at 6 hours,1 days,3 days,7 days after light exposure. The apoptosis of Retinal photoreceptor cells were tested by TUNEL at 1 days after exposure,the morphological change of retinal were viewed by HE at 7 days after light exposure. RESULTS The expressions of microgilal and IL-1 began to rise at 1 days after light exposure,reached at peak at 3 days and decreased at 7 days.The microgial and IL-1 in FTY720 group were higher than normal group,lower than Solvent control group and model group (P<0.05).1 day after exposure to light, the apoptosis cell ratio in normal group, a model group,a Solvent control group and FTY720 group were 0、(87.66±2.50)%、(86.00±2.44)%、(49.66±2.80)%. The apoptosis cell in FTY720 group were higher than normal group,lower than Solvent control group and model group (P<0.05).7 days after exposure to light,the retinal in normal group is structured and the cell is arranged well, the cell in Solvent control group and model group is irregular arrangement and the ONL is thin after light exposure.The thickness of the ONL in FTY720 group was significantly higher than Solvent control group and model group,below normal group. CONCLUSION FTY720 can prevents Retinal photoreceptor cells from apoptosis and inhibits activation of microglial...