Effect Of MUC1 On Biological Behaviour Of Human Colorectal Cancer Cell HCT116

Objective:To investigate the effect of MUC1 on proliferation, invasion and chemosensitivity of human colorectal cancer cell line HCT116 and provide laboratory evidence for MUC1 as the target treatment of colorectal cancer.Methods:1. Established a stable expression of MUC1 HCT116 cell line.(1) Constructed MUC1 lentivirus vector:Found human MUC1 gene by Genebank, selected the appropriate plasmid, amplificated the target gene by PCR, double digested the target gene and lentivirus plasmid, Constructed recombinant plasmid and identified and then transfected 293T cells to package lentivirus.(2) Transfected the HCT116 cells and then selected by puromycin.(3) The expression of MUC1 was detected by SqRT-PCR and Western blot.2. Effect of MUC1 on Biological Behaviour of Human Colorectal Cancer Cell HCT116.(1) There were two groups in our experiment:MUC1 lentivirus group and control lentivirus group.(2) CCK method and soft agar colony formation method were used to detect Cells proliferation.(3) Transwell membrane assay were used to detect cells invasion.3. Effect of MUC1 on oxaliplatin sensitivity:MTT method and flow cytometry were used to detect cells sensitivity to oxaliplatin and the colorimetric method to detect Caspase-3 activity.Results:HCT116 cell line with stable expression of the MUC1 was acquired. Compared with control lentivirus group, the number of soft agar colonies in MUC1 lentivirus group was increased significantly (P<0.05), the number of invasive cells was increased significantly (P<0.05). Chemosensitivity was decreased in the MUC1 lentivirus group (P<0.05). Caspase-3 activity was significantly low in the MUC1 lentivirus group (P<,0.05).Conclusion:MUC1 is related to the anchorage-independent growth, invasion and chemotherapy of colorectal cancer.