Mechanism And Effect Of Shrna-Mediated Silencing SOX2 On Human Breast Cancer Cell Lines MDA-MB-231 Proliferation、Invasion And Apoptosis

Research objective:To know the effect of Targeted gene Sox2 silencing in short hairpin RNA on human breast cancer cell (MDA-MB-231) invasion, proliferation and apoptosis and its possible mechanism.Methods:Construct shRNA recombinant vector of targeted Sox2 gene and transfect it to human breast cancer cell (MDA-MB-231). Then detect the expression of Sox2 mRNA in cancer cells by Q-PCR methods. The apoptosis of cancer cells will be studied by FCM. Transwell method will be introduced to detect cellular invasiveness and metastasis. Cancer cell vitality and proliferation ability will be tested by MTS. The expression of β-catenin andSox2 protein will be detected by using Western blot.Results:Recombinant vector of pMafic7.1-shRNA-Sox2 was constructed. The expression of Sox2 mRNA and β-catenin protein significantly decreased after transfection of pMafic7.1-shRNA-Sox2 to MDA-MB-231 cancer cells. The rate of cell apoptosis clearly increased while its invasive ability, cell metastasis and proliferation decreased. Compared with blank control group (not transfected cells) and negative control group (transfected by pMafic7.1-shRNA-NC cells), there were significant difference between them (P<0.05).Conclusion:Targeted silence gene Sox2 can enhance cancer cell apoptosis, inhibit cell proliferation and invasion. The reason may be its inhibition of Wnt/β-catenin signal path.