Effect Of DEC1 Gene Overexpression On Proliperation And Invasion Abilities Of Human Esophageal Cancer ECA109 Cells

Objective:To investigate the effect of DEC1 gene over-expression on proliferation and invasion abilities of human esophageal cancer ECA109 cells. And to explore its possible mechanisms by the detection of matrix metalloproteinases MMP9 and cyclin cyclinD1 protein expression.Methods:1. ECA109 cells were transfected with plasmid pcDNA3.1 （-）/DEC1 （DEC1 group） and pcDNA3.1 （-） （vector group）,successfully constructed according to the cDNA sequence of DEC1 gene in Genebank （NM₀03670.2）. And divided into the experimental group （pcDNA3.1 group （-DEC1） and group vector （/DEC1） pcDNA3.1 （-））.2. The mRNA levels of DEC 1 were evaluated by real-time PCR after transfection for 48 hours3. The protein levels of matrix metalloproteinase MMP9 and cyclin cyclinDl protein expression were detected by Western blot 72 h after the transfection for 72 hours.4. The effect of DEC 1 over-expression on the proliferation abilities of ECA109 cells were evaluated by CCK-8 assay, colony formation assay5. The effect of DEC1 over-expression on the invasion abilities of ECA109 cells were evaluated by Transwell testRESULTS:1. compared with vector group, the expression of DEC1 in DEC1 group were significantly increased （P<0.01）2. compared with vector group, cyclinD1 DEC1 group, the MMP9 expression decreased obviously （P<0.05）.3. compared with vector group, cell proliferation and invasion ability of DEC 1 group was significantly inhibited （P＜0.01）Conclusion:The over-expression of DEC1 significantly inhibits the proliferation and invasion of ECA109 cells, which may be involved in the expression of cyclin D1 and MMP9...