Polyether Ether Ketone Surface Biological Modificationand Investigation Of Anisotropic Cell Adhesion Microstructure

Biological interface based on the surface of the material has always been an important research topic, it involved in the research field, research methods, and have great significance in practical application. Biological modifying some materials which have excellent performance but process biological inertsurfaces, can greatly broaden application scope of the materials in the field of life science and medicine;Surface patterning of the material surfaces, can make the medical transplant material shows an unexpected effect when implanted organisms. This article based on these background, to sum up have the following two parts: the use of "one-step" will be special plastics polyether ether ketone surface of biological activation on the surface at the same time build nano fiber structure, use of the anisotropy cell adhesion microstructure induced cell migration.The first part, using "one-step" biological activate the surface of special plastics polyether ether ketone and build nano fiber structure at the same time.Polyether ether ketone is a kind of special plastics which has many excellent properties, it has very good application potential in the field of bone graft material owe to its unique biological non-toxic and bone mechanical properties, but the inert surface of the polyether ether ketone biological limits itself.We use of the photo initiation of polyether ether ketone and titanium dioxide, under the condition of uv irradiation, the hyaluronic acid methyl acrylate modified on the surface of polyether ether ketone, at the same time long chain hyaluronic acid methyl acrylate also reacting with the titanium dioxide electrostatic spinning, polyether ether ketone with titanium dioxide electrostatic spinning both together, finally constructed the fiber structure which imitated the bone surface on the surface of polyether ether ketone.Through biological experiments showed that after modification of the surface of the polyether ether ketone has good biocompatibility and the surface which modified of titanium dioxide electrostatic spinning had very good promote osteogenesis stem cells differentiate ability. The biological interface method which we design has a very good reference for application of polyether ether ketone as bone graft material.The second part, using of the anisotropic cell adhesion microstructure induced cell migration.Cell migration is a common and important behavior for cells, such as embryonic development, wound healing, and tumor cells transfer process are all relating to cell migration behavior.Constructing controllable induced cell migration interface on the material surface has great significance for research and biological implant material.We constructed PDMS surface by means of duplicating a micro column structure, then modifying the PEG molecular which blocked cell adhesion in PDMS surface, by means of inclined evaporation deposition in the side of the micro column 20 nanometers thick layer of gold nanoparticles, then modifying the RGD peptides which promoted cell adhesion on the gold surface, finally contrusting the anisotropic cell adhesion microstructure.We conveying the asymmetry characteristics of the anisotropic to the cells adhesion through the surface patternning, so that the cells asymmetric adhesion caused cells "polarization" directional migration behavior.Through the experimental results show that we are building the microstructure has the function of directional induced cells and the microstructure can help to study the migration behavior of the various cell mechanism and regulation of tumor cell organisms spread which all have great significance in practical application.