Experimental Study On The Influence Of CO₂ Pneumoperitoneum On Bacteria Peritonitis In Rats

Objective: To study the effect of different carbon dioxide pneumoperitoneum pressure and time on abdominal cavity infection bacteria of peritonitis in rats, including bacteria growth and bacterial translocation. To detect carbon dioxide pneumoperitoneum promoted the bacteriagrowth and translocation in peritonitis. Discussing the CO2 pneumope-ritoneum influenced on bacteria of peritonitis antibiotic drugs sensitivit-y.Methods: 60 Sprague Dawley rats were injected with Eseherichia coli into the abdominal cavity to establish models of intra-abdominal infection and randomly divided into six group by random number table.Four groups with carbon dioxide pneumo-peritoneum(5-15 mmHgfor 1h-3h), and two control group with no pneumoperitoneu-m for 1h-3h. All rats were killed at the end of the carbon-dioxide pneumo-peritoneum experiment. Peritoneal lavage fluids and portal vein blood were taken for microbiological examinations and culture. The endotoxin content in portal vein blood was detected.And to detecte antibiotics sensitivity experiments of e-coli isolated form peritoneal.Results: After three hours pneumoperitoneum, high pressure group and low pressure group compared with control group, the concentr-ation of Colony Forming Units of bacteria increased obviously(P<0.05). In addition, high pressure group blood culture positive rate is higher than the control group(P<0.05). The endotoxin content in plasma increased obviously(P<0.05) in high pressure group compar-ed with low pressure group. And the endotoxin content in plasma increased obviously(P<0.05) in low pressure group compared with control group. The escherichia coli of peritoneal in every group by pneumo-peritoneum, whose antibiotics bacteriostasis circle diameter were no difference(P>0.05). There were lots of intestinal common aerobic and anaerobic bacteria in peritoneal lavage fluid and blood culture.Conclusion: Carbon dioxide pneumoperitoneum promoted intestinal bacterial endotoxin and bacterial translocation in peritonitis of rats, and they were increased with the pressure and time. Carbon dioxide pneumoperitoneum environment promoted the growth of anaerobic bacteria in peritoneal so as to the total number of bacteria increased significantly than without pneumoperitoneum. Abdominal infection was given priority to mixed bacteria infection, and Carbon dioxide pneumoperitoneum for a short period of time has no effect on bacterial drug resistance.