| BackgroundGastric cancer is one of the most common malignant gastrointestinal tumors, although its worldwide incidence has a downturn.However, in most Asian countries, the incidence of gastric cancer remains stubbornly high. China is one of areas with high incidence of gastric cancer,the incidence of which ranges secondly and the mortality, ranges thirdly, seriously affecting the quality of life of people of our country. Therefore, the fight against gastric cancer is still the problem eargely to be solved. Chemotherapy is an important means of treatment of gastric cancer, mainly for patients not suitable for surgery and prevention of postoperative relapse. However, due to gastric cancer pathogenesis complexity, serious adverse reactions and multi-drug resistance, the effect of chemotherapy has been severely limited. Therefore, finding a significant effect of chemotherapy still has important significance. In recent years, tetrandrinewas concernedgradually as chemotherapy drugs used in cancer therapy.ObjectiveIn this paper, the aim was to study the inhibitory effect of tetrandrine on cultured gastric cancer cell line MGC 803 and its mechanism, and discuss the valueof tetrandrine in gastric cancer chemotherapy.MethodsMTT assay wsa used to study inhibition of tetrandrine on proliferation of clutured gastric cancer cell line MGC 803 of and the median inhibitory concentration (IC50) wsa calculated; Impact of colonyformation ability of tetrandrine on gastric cancer cell line MGC 803 cells wsa tested using crystal violet staining assay; Hoechst 33258 staining wasused to detect change of MGC 803 nucleus after treatment of tetrandrine; Useing AnnexinV-FITC/PI staining to detect apoptosis inducing effect of tetrandrine by flow cytometry on MGC 803 gastric cancer cells; PI staining was used tb detect cell cycle distribution of gastric cancer cell line MGC 803treated with tetrandrine by flow cytometry; influence of tetrandrinon mitochondrial membrane potential of MGC 803 cells was deteced using JC-1 staining assay; DCFH-DA fluorescent probe staining wsa used to analyze reactive oxygen content within the cell line MGC 803 cells tetrandrine gastric cancer; Transwell method study was preformed to test the impact on the migration of gastric cancer cell line MGC 803 cells; western blot was used to study the mechanism of tetrandrine on MGC 803 gastric cancer cells in vitro.Results(1) MTT results showed that tetrandrine inhibited proliferation of human gastric cancer MGC 803 cells in a time and concentration-dependent mannerin vitro.(2) Colony formation assay showed that trtrandrine inhibited proliferation of single MGC 803 cell.(3) Hoechst 33258 staining showed that after tetrandrine treatment of gastric cancer MGC 803 cells, with the increase of drug concentration, the gradual emergence of the nucleus shriking, suggesting apoptosis.(4) Flow cytometry results showed that tetrandrine can induce a concentration-dependent apoptosis in gastric cancer MGC 803.(5) Flow cytometry showed that tetrandrine induced cell cycle arrest at G2/M phase on gastric cancer MGC 803 cells in a concentration-dependent manner.(6) JC-1 staining showed a decline of mitochondrial membrane potential after tetrandrine treatment in gastric cancer cells MGC 803.(7) DCFH-DA staining showed that the level of ractive oxygen content in gastric cancer cell MGC 803 cells increased after treatment tetrandrine.(8) Transwell assay showed that tetrandrine inhibited the migration of gastric cancer cells MGC 803.(9) Western blot analysis showed that the treatment of tetrandrine upregulated p53, Bax, cleaved-PARP and down-regulated Bcl-2 and N-cadherin of MGC 803 gastric cancer cells.ConclusionTetrandrine can inhibit the proliferation and migration of gastric cancer MGC 803 cells and may be a potential stomach cancer chemotherapy drug. |
PDF Full Text Request