Establishment Of A Hypoxia-induced Rat Model And The Effect Of Emodin On Hypoxic Brain Edema

Background and ObjectiveThe human brain requires a certain concentration of oxygen to maintain normal physiological function, any factors that cause neonatal hypoxic hypoxia can lead to brain damage, suffocation caused by hypoxic-ischemic encephalopathy is a common serious complication of pediatric abroad; In addition, national defense needs and the state of implementation of the western development policy makes people living in high altitude hypoxia areas, work and other activities will also be increasingly widespread, strengthen research on the characteristics of hypoxic brain injury damage, and to hypoxic brain edema prevention and treatment has important practical significance.Animal models are an important part of scientific experiments, development of new production hypoxic animals JEM means for hypoxic brain edema has a crucial role in how to build a reliable and stable hypoxic brain edema animal model is a difficulty, we focused on technical deficiencies exist in the past, research and development production of new hypoxic animals JEM means success in animal models of cerebral edema under hypoxic conditions.Emodin is an important component of people extracted from rhubarb, my research group found in previous studies on brain tissue emodin and blast injuries caused by epilepsy in rats has a protective effect. In this study, we establish stable hypoxic brain edema model in rats to explore emodin on brain tissue and its mechanisms, provide a theoretical basis for clinical application.Methods1. Hypoxia animal JEM and exhaustive exercise devices utilize research group designed to establish animal models of hypoxic brain edema in rats were observed during hypoxia physiological changes in the behavior of rats, pathological changes in the brain tissue HE staining, the use of stem Determination of brain water content of wet weight method.2. Hypoxic brain edema emodin observed in hypoxic rats survive status JEM, using Morris water maze automatic image acquisition and processing system of rats behavioral test, measurement of brain tissue containing wet and dry weight method water.Result1. The model of hypoxic cerebral edema of rats is made by this method, the rats in the animal experimental cabin of low oxygen are observed to have apathetic spirit, slow behavior, poor diet, poor appetite, and respiratory rhythm change, and different degrees of respiratory arrest phenomenon, and the serious ones have convulsions, death and other symptoms.2. Establish stable animal model of hypoxic cerebral edema of rats for carry out the emodin intervention experiment to the rat: the rats in all groups in the animal experimental cabin of low oxygen have shown different extends of slow deep breathing, pause and other respiratory rhythm changes, as well as limbs twitch, listlessness, poor appetite and slow behavior. The cerebral cortex is congested, and the brain tissue appears edema. The pathological sections of brain tissue were observed under light microscope. The pathological changes of brain tissue were observed, and the space between cells and blood vessels is increased, and the nerve fibers were loose.3. The emodin has clear brain protection function on hypoxic cerebral edema; in the water maze test, the emodin intervention group has obviously reduced time and trip in the process of seeking water maze hidden platform than that of the hypoxia group, the water content of brain of after-injury hypoxia group and emodin intervention group is significantly higher than those in the control group. The water content of brain tissue of emodin intervention group is reduced relative to that of the hypoxia group. The content of MDA in brain tissue is decreased relative to that of the hypoxia group, and the activity of SOD was decreased relative to the hypoxia group.The water content of brain tissue increased, and the water content of cedebral edema increased significantly with the increase of the degree of hypoxia.4. Use the wet/dry weight method to measure the water content of brain tissue of rats, it is noted that the water content of brain tissue was increased at different extent in comparison with that of the control group, as the increase of hypoxia group, the water content of cedebral edema is increased significantly.5 General observation under pathologic section: Cut the brain tissue samples on the right side, after fixation, washing, dehydration and paraffin wax treatment, slice by using the slicing machine, make the tissue section, and observe the tissue pathological change by staining with hematoxylin and eosin. The cerebral cortex was filled with congestion and edema in each group. In the case of high magnification, the neurons were found to have edema. As the increase of hypoxia degree, the levels of blood vessels and nerve cells are increased gradually. The gap of the blood vessel was gradually increased; and there has no abnormal change and the structure is complete under the observation of rat brain tissue experimental glasses.Conclusion1. The low-pressure low-oxygen animal experimental cabin is attached with an animal swimming tank and established with a stable, reliable and repeatable animal model of hypoxia induced brain injury of rats.2. The emodin can reduce the pathological injury of brain tissue, that has a protective effect on hypoxic cedebral edema of rats.