The Mechanism Of Astragaloside Ⅳ Protects Cardiomyocytes Against Anoxia/Reoxygenation Injury

Objective:Chinese medicine Huangqi has been used in the treatment of many cardiovascular diseases. Astragaloside Ⅳ is one of saponins, which has much pharmacological activity. We established the cultured primary neonatal rat myocardiocytes with the acute myocardial A/R injury model to explore the mechanism of the protective effect of Astragaloside IV on cardiomyocytes anoxia/ reoxygenation injury.Method:The cultured newborn rat of two or three days myocardiocytes were randomly divided into four groups, each group repeated four times:(1)Control group; (2) A/R group; (3) AsIV+A/R group; (4) AsIV+ABT-737+A/R group. The cell viability was detected by MTS; Western blotting analysis assayed the expression of bcl-2; The DCFH-DA assayed the level of intracellular reactive oxygen species ROS; According to the kit,the activity of SOD, LDH, and GSH-Px was analyzed with an automatic biochemical analyzer;Results:1.The expression of Bcl-2 protein after processing A/R were increased compared to control group but compared with A/R group, the AsIV+A/R group were significantly reduced (P<0.01).2. a. After A/R, the content of MDA was raised, the activities of SOD and GSH-Px were lower and LDH was higher, increased the level of ROS, the mitochondria membrane potential was markedly decreased, and the apoptosis was higher than control group, and all indicators were significant differences (p<0.01); b. After AsⅣ+A/R, the cell viability was increased, the contents of MDA was obviously lower, the activities of SOD and GSH-Px were higher and reduce the LDH, decreasing producion of ROS, the stability of mitochondria membrane increased, and the apoptosis was decreased, compared to A/R group, and all indicators were significant differences (p<0.01); c. Compared AsIV+A/R group with AsIV+ABT-737+A/R group, all indicators were significant differences (p<0.01),but no significant differences when A/R group compared to AsIV+ABT-737+A/R group(p>0.05).Conclusion:Astragaloside IV can upregulating the protein expression of Bcl-2 fight against anoxia/reoxygenation damage.