Application Of Fe₃O₄ Magnetic Nanoparticles On The Separation Of Ingredient In Traditional Chinese Medicine

Our country has become the world’s largest producer of herbs due to the vast land area, we have thousands of years of clinical experience and civil application history in Chinese traditional medicine research. Unfortunately,the majority work for China to develope Chinese herbal medicine is exporting raw materials,and the development of advanced products have been in a bottleneck for the technical level restriction. So,the road for our country in Chinese medicine research and developmenty is still very difficult.At present, the traditional separation methods not only operate complexly and pollute the environment, but also take great labour forces, materials and financial resources, which can not meet the large-scale and deep-seated traditional Chinese medicine research and development needs. Therefore,developing an new efficient and economic separation technology is imminent.The Fe3O4 magnetic nanoparticles as a new type of nano-materials, have specific adsorption functionality on the ortho phenolic hydroxyl and carboxyl. As this adsorption way, you can use magnetic nanoparticles as a intermediate carrier to separate components of traditional Chinese medicine, thus achieving the purpose for rapid purifying Chinese medicines.This paper cover the following aspects:1.The Fe3O4 magnetic nanoparticles were synthesized by coprecipitation, and characterized by fourier transform infrared spectrometer(FTIR) and dynamic laser light scattering apparatus(DLS).The Fe2 + / Fe3 + ratio, reaction time, stirring speed, save solvent and other conditions have been researched, to obtain a good dispersion, uniform size of nanoparticles. Determining the Fe3 + / Fe2 + ratio of 1.8: 1, stirring rate of 600 rpm, 20 min reaction time are the optimum conditions for the preparation of nanoparticles.2.With magnetic nanoparticles as a carrier,which have specific adsorption of the carboxyl group, we separated and purified the Ginkgo biloba acid from alkyl phenols compounds successfully. By optimizing experimental conditions,we ultimately determined to use methanol as the adsorption solvents,and volume ratio of 10% formic acid as the eluent in desorption program in acetonitrile solution, effectively saving the solvents and raw materials.The structure of the isolated ginkgo acid have been characterized by Fourier transform infrared spectroscopy(FTIR), High performance liquid chromatography-ultraviolet(HPLC-UV), Electrospray ionization mass spectrometry(ESI-MS / MS), Nuclear magnetic resonance(NMR).3.By adsorptive study of Scutellaria and liquorice, we confirmed the selective adsorption of magnetic nanoparticles for flavonoids is caused by ortho phenolic hydroxyl and carboxyl.By optimizing the chromatographic gradient conditions, mobile phase conditions,we ascertained the best chromatographic analysis method, and further confirmed the solution with nanoparticle volume ratio of 5: 1 is optimal adsorption condition. Simultaneously,the eluent pH value of 1 is the best solution desorption condition.Finally,we established the new separation and purification methods for flavonoids and triterpenoids by using magnetic nanoparticles as a adsorption carrier.