| Objective:China is one of the countries with high morbidity and mortality of esophageal cancer in the world, the death toll of esophageal cancer in China has accounted for more than half of the world(52.8%).Esophageal cancer is mainly composed of squamous cell carcinoma in China, and its pathogenesis is still unclear. Therefore, searching for effective molecular biological control targets and providing reliable theory basis for early detection and treatment of esophageal cancer become the research focus.Following the deep study in the pathogenesis of tumor in recent years, it reveals that small RNA(mi RNA) can control tumor related genes, which has the effect of oncogenes or tumor suppressor genes. It is found that many mi RNA cells are involved in the differentiation,proliferation and apoptosis of cells, so as to control the occurrence,development and metastasis of esophageal squamous cell carcinoma.The mi RNA-193 b and the mi RNA-199 a play different functions in gastric cancer, colon cancer, endometrial cancer and other tumors. But whether they are involved in the occurrence and development of esophageal squamous cell carcinomas is still unclear and has not yet been reported.This study detects the expression of mi RNA-193 b and the mi RNA-199 a in 56 cases of esophageal cancer tissue and corresponding normal tissue adjacent to carcinoma, and analyzes its relationship with clinicopathologic feature of patients with esophageal squamous cell carcinomas, explores the clinical significance of mi RNA-193 b and mi RNA-199 a in esophageal squamous carcinoma.Methods:1 Select esophageal carcinoma primary tumor tissue and adjacent tissues of patients with esophageal squamous cell carcinoma and treated by surgical treatment from 2014 to 2015 in fourth hospitals of Hebei Medical University. Total number is 56 cases. Avoid tumor necrosis area when collecting primary lesion. Tissue adjacent to carcinoma is normal tissue of more than 5 cm distance to tumor tissue(pathological confirmed). Time for all specimens in vitro is less than 30 min. Cut the specimen into tissue blocks at sizes of around 1 cm3, put them into liquid nitrogen to frozen for 2 ~ 3 min, then archive them in- 80 ℃. Collect the clinical data of the patients, including age, gender, pathological results, etc. and stage according to UICC seventh edition of esophageal cancer TNM criteria.2 Use technology of "Green q RT-PCR SYBR" to detect expression levels of mi RNA-199 a and mi RNA-193 b in esophageal squamous cell carcinoma and adjacent tissues. Explore the relationship between mi RNA-193 b, mi RNA-199 a and esophageal squamous cell carcinoma based on clinical pathological data.3 SPSS19.0 is applied to analyze the results of experiments.Results:1 The expression of mi R-193 b in esophageal carcinoma and its relationship with clinical pathological data1.1 Expression of mi R-193 b in esophageal carcinomaIn 56 cases, the expression of mi R-193 b of esophageal carcinoma tissue is 0.052±0.004, while the expression of the tissue adjacent to carcinoma is 0.089±0.005; The expression of mi R-193 b in esophageal carcinoma tissues was significantly lower than that in adjacent tissues,there is a significant difference between the two, and has statistical significance(t=-30.972, P=0.000).1.2 Relationship between mi R-193 b expression and clinical data in esophageal carcinomaAccording to the clinical and pathological data of patients withesophageal cancer,the expression of mi R-193 b is associated with TNM stage(P<0.05), no significant association with age, gender, tumor differentiation and lymph node metastasis(P>0.05) was found. The expression of III+IV is 0.050±0.010, significantly less than of patients with I+II(0.055±0.003), and has statistical difference(t=6.112,P=0.000).2 The expression of mi R-199 a in esophageal carcinoma and its relationship with clinical pathological data2.1 Expression of mi R-199 a in esophageal carcinomaIn 56 cases, the expression of mi R-199 a in esophageal carcinoma tissue is 0.056±0.006, while the expression of tissue adjacent to carcinoma is 0.082±0.004; The expression of mi R-199 a in esophageal carcinoma tissues was significantly lower than that in adjacent carcinoma tissues, there is a significant difference between the two, and has statistical significance(t=-36.222, P=0.000).2.2 Relationship between mi R-199 a expression and clinical data in esophageal carcinomaAccording to the clinical and pathological data of patients with esophageal cancer, the expression of mi R-199 a is associated with tumor differentiation, TNM stage and lymph node metastasis(P<0.05), no significant association with ager and gende(P>0.05) was found. The expression level of the patients with low differentiation is 0.054±0.004,which is significantly lower than that of the middle and high differentiation(0.058±0.009), and has statistical difference(t=3.498,P=0.005); The expression of III+IV is(0.052±0.011), significantly lower than that of patients with I+II(0.058±0.007), and has statistical difference(t=5.19, P=0.000). The expression level of patients with lymph node metastasis is(0.055±0.008), which is significantly lower than that of patients with no lymph node metastasis(0.058±0.009). and has statistical difference(t=3.064, P=0.007).Conclusions:1 The expression of mi R-193 b in tissues of esophageal squamous cell carcinoma is much lower than those of the adjacent tissues, and it is significantly correlated with TNM staging. This suggests that mi R-193 b may be a potential tumor suppressor gene in esophageal squamous cell carcinoma and plays an important role in the occurrence and development of esophageal squamous cell carcinoma.2 The expression of mi R-199 a in tissues of esophageal squamous cell carcinoma is much lower than those of the adjacent tissues, and it is significantly correlated with tumor differentiation, TNM stage and lymph node metastasis. This suggests that mi R-199 a may be a potential tumor suppressor gene in esophageal squamous cell carcinoma and plays an important role in the occurrence and development of esophageal squamous cell carcinoma. |
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