Effects Of Resistin On AMP-activated Protein Kinase Signaling Pathway In Spontaneous Hypertensive Rat

ObjectiveTo investigate the influence of resistin on AMP-activated protein kinase (AMPK) in spontaneous hypertensive rat (SHR).MethodsIn the present study, the rat model was set up by injection of recombinant adenovirus expressed resistin. And the effects of resistin on its systolic blood pressure and adiponectin signaling pathway were also observed. The 8-week-old spontaneously hypertensive rats were separated into SHR group, high-dose resistin group, medium-dose resistin group, low-dose resisitin randomly. The 8-week Wistar rats were used as control. Tail artery systolic pressure of every rat was recorded the day before the injection and 4 weeks after injection. The rats were sacrificed 4 weeks after injection. The systolic pressure and index of myocardial hypertrophy were examined. The level of serum adiponectin was detected by ELISA. RT-PCR was used to measure the expression of Adipo R1 mRNA. The AMP-activated protein kinase was evaluated by Western blot.ResultsCompared to Wistar rats, the systolic pressure and index of myocardial hypertrophy in SHR were significant increased (P<0.05). And on the other hand, the expression of adiponectin, Adipo Rland AMPK were reduced (P<0.05). In the SHR groups, positive correlation between the doses of resistin and systolic pressure, index of myocardial hypertrophy were also found. However, negative correlation were noticed between the doses of resistin and the expression level of AN, AdipoRl, AMPK.ConclusionThe myocardial hypertrophy of SHR probably related to systolic pressure and adiponectin. Resistin could reduce the adiponectin, and at the same time it could also decrease the expression of AdipoRl and AMPK, which probably could be one of the molecular mechanisms to induce the changes of myocardial metabolism.