Effects Of Combination Of Treadmill Exercise And Massage On Inflammation Development And Satellite Cell Proliferation Inrehabilitation Process Of Ratsacute Skeletal Muscle Injury

Objective To investigate the effect of the combination of treadmill exercise and massage on the inflammationNADH of the damage tissue and the expression of nNOS、HGF mRNA in the satellite cell proliferation on the rehabilitation process of ratsacute skeletal muscle injury so that to explore the role of massage on therehabilitation to acute skeletal muscle.Methods 76 adult male SD rats were randomly divided into 5 groups: normal control (group A,n=4),self-healing group (group B,n=20),control group of massage (group C,n=16),control of treadmill exercise (group D,n=16),control of mixture(group E,n=16),.In group A,nothing were done;In group B、C、D and E, rats were made to the model of rats with acute muscle injury by the improved traumatizing unit.Group B were not given the treatment,massage were given to the group C、E and treadmill exercise were given to the group D、E after three days of the modeling.At 7,14,21,28days after the modeling,animal gastrocnemius samples were token in group B、C、Dand E respectively,HE staining was employed to inspect pathological changes.the inflammationNADH was detected by the Western Blot, Real-time PCR was applied to detect the expression of nNOS, HGF mRNA in muscle satellite cells.Results After the combination interventionof massage and treadmill exercise,the expression of NADH,nNOS, HGF mRNA was higher in group C、D、E than the groupB,and it has highly significant differences (P<0.01).Compare to the group C and D,the expression of NADH,nNOS, HGF mRNA was more in the group E,and it has highly significant differences (P<0.01).Compare to the group C,the expression of nNOS HGF mRNA of in Group D was higher,and it has significant differences (P<0.05).HE staining shows that myofilaments were disorder and ruptured,inflammatory cells were infiltrated,groud A was not. Compare to the group B,group C、D、E has more blood supply, more myofilaments, more activated muscle satellite cells, necrotic tissue wasrehabilitated faster compare to group B.Conclusion The combination of treadmill exercise can effectively improve the expression level of the NADH、nNOS, HGF mRNA in the muscle satellite cells, promote the inflammation to fade, the proliferation of satellite cell, provide the speed of the rehabilitation and regeneration in the damaged skeletal muscle...