The Expression And Significance Of β-HCG,ERK1/2 And MMP-2 In The Advanced Epithelial Ovarian Cancer

Objective: To research the impact and the mechanism of β-HCG,ERK1/2,p-ERK1/2 and MMP-2 about the epithelial ovarian cancer.Method:1.Collected the paraffin and patients,clinical data of 24 cases of advanced(FIGO ~ period) Ⅲ Ⅳepithelial ovarian cancer and 20 cases of normal ovaries that resection for benign gynecologic disease.Immunohistochemical staining was used to determine the expression of β-HCG,ERK1/2,p-ERK1/2 and MMP-2,tissue microarray slices obtained from including the tissue of ovarian tumor and metastatic sites tumor with the paraffin of advanced epithelial ovarian cancer,the paraffin of normal ovarian tissues made to conventional slice.2. Chi-square test and Fisher’s exact probability method is used to analyze the correlation about β- HCG, ERK1/2,p- ERK1/2 and MMP- 2 in advanced epithelial ovarian cancer occurrence, metastasis and differentiation. The Spearman rank correlation analysis of the relationship that the expression level of β-HCG with ERK1/2,p- ERK1/2and MMP- 2 in advanced epithelial ovarian cancer. Significance level definition of α = 0.05, P < 0.05 were considered statistically significant.Results:1.β-HCG mainly expression in cell membrane and cytoplasm of advanced epithelial ovarian cancer.In the tissue of ovarian tumor and metastatic sites tumor,the positive expression rate of β-HCG were 54.2% and 83.3%,in normal ovarian tissue,only one case expressed weakly positive of β-HCG. ERK1/2 mainly expression in cell cytoplasm and little expression in cell nucleus of advanced epithelial ovarian cancer. In the tissue of ovarian tumor and metastatic sites tumor, the positive expression rate of ERK1/2 were58.3% and 79.2%, in normal ovarian tissue, ERK1/2 expressed negative. In the tissue of ovarian tumor and normal ovarian tissue, p-ERK1/2 expressed negative, in the tissue of metastatic sites tumor, only one case expressed weakly positive of p-ERK1/2.MMP-2 mainly expression in cell cytoplasm of advanced epithelial ovarian cancer. In the tissue of ovarian tumor and metastatic sites tumor,the positive expression rate of MMP-2 were 66.7% and 91.7%, in normal ovarian tissue,only two case expressed weakly positive of MMP-2. In advanced epithelial ovarian cancer, the positive expression rate of β-HCG,ERK1/2 and MMP-2 were higher than in normal ovarian tissue and contrast the differences were statistically significant(P<0.01, P<0.01, P<0.01).2.The positive expression rate of β-HCG,ERK1/2 and MMP-2 in the metastatic sites of tumor tissue were higher than the ovarian tumor tissue, the differences were statistically significant(P<0.05, P<0.05, P< 0.01).3.In the poorly differentiated tumor tissues that ovarian parts, the positive expression rate of β-HCG,ERK1/2 and MMP-2 were 78.6%,85.7% and 92.9%, in the high, middle differentiation group that ovarian parts, the positive expression rate of β-HCG,ERK1/2 and MMP-2 were 20.0%,40.0% and 10.0%. The positive expression rate of β-HCG,ERK1/2 and MMP-2 in the poorly differentiation tumor tissues that ovarian parts were higher than the high, middle differentiation group, the differences were statistically significant(P<0.05, P<0.05, P<0.05).3. β-HCG, ERK1/2 and MMP-2 have nothing to do with the patient’s age and patient’s age and ovarian tumor size, there were no statistically significant difference.4.The expression of β-HCG with ERK1/2 and MMP-2 were positively correlated in advanced epithelial ovarian cancer(P<0.05,P<0.01,P<0.01,P<0.05).Conclusion: 1. β-HCG might promoted occurrence and progress of epithelial ovarian cancer, 2.β-HCG could promoted the occurrence and progress of epithelial ovarian cancer by activating the related signaling pathways about ERK1/2-MMP-2.