Effects Of MiRNA-423-5p On Trefoil Factor1 Expression And On Biological Behavior Of Gastric Mucosal Epithelial Cells

Background: Trefoil factor family is a group of peptides synthesized and secreted by mucosal epithelia. TFFs has important biological function in mucosal lesions, chronic inflammation and tumorigenesis development. They are potential tumor markers, and its expression and regulation is of great significance to the diagnosis and treatment of gastrointestinal tumor. TFF1 has important roles in the repairing of the epithelium after mucosal injury, and is associated with cell proliferation, apoptosis and migration in epithelial cells. micro RNA has a key role in regulating gene expression network and take part in multiple signaling pathways. mi R-423-5p exerted its biological function by negatively regulating the expression of TFF1, which was previously identified as its downstream target gene.Aim: Here we further examined the roles of mi RNA423-5p in gastric mucosal epithelial cells by down-regulating TFF1.Methods: First of all, mi RNA-423-5p expression in GC and normal tissues and cells was examined by q RT-PCR, IHC and Western blot. Then, human gastric cells GES-1 was examined after transfecting with mi RNA-423-5p mimics. We peformed MTT, Ed U, and colony formation assay detecting the ability of proliferation. Laser scanning confocal microscope was used for assessing cell apoptosis. Wound healing and Transwell were applied to evaluating the cell migration.Results: We showed that mi R-423-5p expression is low in normal human gastric cells and tissues. However, TFF1 is high expression in normal human gastric cells and tissues. Over-expression of mi R-423-5p by transfecting mimics oligos into human gastric cells GES-1, decreased the expression of TFF1, and further promoted cell proliferation but inhibited cell apoptosis and migration.Conclusions: Our results strongly suggested that via a TFF1-dependent manner mi RNA423-5p may take part in the regulation of cell proliferation, apoptosis and migration in gastric epithelial cells. The present study provides evidence that mi RNA-423-5p contribute to the repairing of gastric mucosa.