Experimental Study On The Effect Of Sodium Hydrosulfide On Bone Cancer Pain In Rats With SD

Objective to observe sodium hydrosulfide(Na HS) effect on bone cancer pain in rats with SD hot pain behavior, spinal microglia activation and cell factorgene expression TNF-α, IL-6 and to explore its possible mechanism in rat.Methods 90 female SD rats, 50-180 g using the random number table method was divided into six groups, each group of 15 only(control group, sham operation group, BCP group, Na HSs group, Na HSM, Na HSH group). There was not any operation treatment in control group, sham operation group inject saline into tibial medullary cavity, BCP group inject Walker256 cancer cells into tibial medullary cavity and Na HS groups based on the model of BCP by intraperitoneal injection of rats in three different doses of sodium hydrosulphide(Na HS). The rats were measured with thermal paw withdrawal latency(PWTL) and immunohistochemistry was used to detect the expression of OX-42 in the spinal dorsal horn of rats and the levels of IL-6 and TNF-α were detected by using RT-PCR. P < 0.05 was statistically significant, within the statistical methods using single factor analysis of variance, multiple comparison, between groups and groups.Results(1) Pain behavior change: preoperative rat hind legs PWTL on both sides of differences between all groups had no statistical significance(P > 0.05); Compared with CT group, there was no differences in the Sham group rats PWTL(P > 0.05); Compared with the Sham group, PWTL of BCP group rats of the postoperative 1d, 3d, 5d, 7d, 10 d, 14 d, 18 d and 21 d of operation side hind limbs was decreased by the time dependent almostly, and especially on the postoperative 14 d, PWTL was significantly decreased(29.77±0.31 s vs 11.3±0.87 s, P < 0.01); Na HS could significantly improve SD rats PWTL of BCP depending on dose and PWTL of Na HS中 group was significantly higher than the BCP group, respectively(PWT: 17.16±0.64 s vs 11.3±0.87 s, P<0.01),(2) The Immunohistochemical immunohistochemical staining results showed that there was no statistical differences of the rats spinal cord dorsal horn microglial OX-42 expression between CT group and Sham group about rats spinal cord dorsal horn microglial OX-42 expression(P> 0.05); Compared with Sham group, the expression of OX-42 in dorsal horn of spinal cord in rats of BCP group was increased with time, and the expression of OX-42 was significantly increased on the fourteenth days after operation(187.44±2.82 vs 66.89±0.23, P<0.01);Compared with the BCP group, Na HS significantly reduced the BCP rat spinal cord dorsal horn OX-42 expression level and the OX-42 expression level of the Na HS中 group decreased significantly(110.17±2.86 vs 187.44±2.82, P<0.01),(3) RT-PCR showed that the IL-6 and TNF-α gene expression levels in the rat spinal cord dorsal horn of CT group and Sham group were no significant differences(P > 0.05); Compared with the Sham group, the IL-6 and TNF-α gene expression level in the rat spinal cord dorsal horn of the BCP group increased significantlyand respectively(IL-6: 0.869±0.025 vs 0.029±0.002, P<0.01; TNF-α: 1.539±0.145 vs 0.087±0.008, P<0.01); Na HS could decrease the IL-6 and TNF-α gene expression of BCP rats by the dose dependent and compared with the BCP group, the IL-6 and TNF-α gene expressions of the Na HS中group were significantly lower and respectively(IL-6: 0.252±0.021 vs 0.869±0.025, P<0.05; TNF-α: 0.29±0.086 vs 1.539±0.145, P<0.05).Conclusion The mechanism of Na HS mitigation bone cancer pain in rats with SD may be related to the inhibition of the spinal dorsal horn microglia activation and reducing the expression of inflammatory factors IL-6 and TNF-α.