Eeffect Of PPARγ Agonist On Cholesterol Content And The Expression Of ABCG1、LXRαin RAW264.7 Macrophage-derived Foam Cell

Objective:By cultivating RAW264.7 mice macrophage in vitro, established a model of foam cell, to measue influence of PPARγ agonist pioglitazone hydrochloride(pioglitazone hydrochloride, PG) on foam cell cholesterol content and the expression of ABCG1, LXR alpha.The aim is to preliminary explore the regulatory mechanism of PG in cholesterol in the foam cell. Methods:1. RAW264.7 mice macrophage cultivated in vitro, incubated by 50 mg/L OX-LDL 48 h, finally oil red O stained and observed cell morphology and change under light microscopy.2.With different concentrations of pioglitazone hydrochloride that control group 0mol/L, and the experimental group(including 5 mol/L, 10 mol/L, 20 mol/L, 30 mol/L) after 24 h, enzyme colorimetry to detect the content of cholesterol in the cell foam;At 30 μmol/L pioglitazone foam cells at different times Oh(control group, experimental group 6 h, 12 h, 24 h, 48 h), enzyme colorimetry to detect bubbles cholesterol in the cell.3.Will RAW264.7 mice macrophage foam cells that give different concentrations of pioglitazone hydrochloride action after 24 h, rt-pcr and Western blot were determined ABCG1, LXR alpha gene and protein expression level. Results:1.In vitro culture of mouse RAW264.7 macrophages adherent growth, mainly in class, round and irregular polygon cells are smaller and contain one or two nuclear, has the pseudopod extend, the 50 mg/L OX- LDL, after 48 h incubation cells significantly larger, oil red O staining of red dye can be seen in the cytoplasm lipid drops.2.Compared with control group, after 24 h the different concentrations of pioglitazone hydrochloride acted on RAW264.7 mice macrophage derived foam cell, intracellular cholesterol ester content were significantly decreased(P < 0.01); As well as,RAW264.7 mice macrophage derived foam cell roled by 30 mmol/L pioglitazone hydrochloride in 0 h, 6 h, 12 h, 24 h, 48 h, intracellular cholesterol ester content were significantly decreased(P < 0.01).3.Compared with the control group, ABCG1, LXR alpha m RNA and protein levels were positively correlated with concentration of PG(P < 0.01). Conclusion:(1) PPARγ agonist can reduce RAW264.7 mice macrophage derived foam cell cholesterol levels, and a concentration and time dependence.(2)PPARγ agonist can increase concentration dependence RAW264.7 mice macrophage derived foam cell ABCG1, LXR alpha gene and protein expression.(3)PPARγ agonist to reduce the content of cholesterol ester foam cell may be done by raising ABCG1, the expression of LXR alpha, it may also be one of the main mechanisms reduce atherosclerosis(astherosclerosis, AS).