Correlation Of Different Doses Of Ceftriaxone And Kidney Stone Formation In Rats

Objective:Observed in rats using different doses of ceftriaxone and the relevance of the formation of kidney stones,to further explore the mechanism of drug-induced formation of kidney stones.Methods:The 40 SD rats were randomly divided into four groups: control group(A), the normal dose Ⅰ group(B), the normal dose Ⅱ group(C), high-dose group(D). A group control group, group B intravenous injection 50 mg / Kg / d ceftriaxone solution, group C intravenous injection of 100 mg / Kg / d ceftriaxone solution, D group intravenous injection of 150 mg / Kg / d ceftriaxone pine sodium. Experimental rats were sacrificed on day 10, remove the kidney tissue, after HE staining paraffin right kidney, the kidney was observed under a microscope whether the crystals form. Left kidney tissue preparation, detection of the left renal tissue superoxide dismutase(SOD) Vigor and malondialdehyde(MDA) Indexes, renal crystal deposition was observed under a microscope.Results:During the experiment, the rats normally live, no deaths. A, B, group C, no significant crystal formation; D team found that within three rat kidney crystals form,representing A, B, group C, the difference between the groups was statistically significant(P <0 05.). A,B,C,D four groups of the left kidney of rats were measuredSOD activity(184.04 ± 13.66),(170.81 ± 18.36),(162.89 ± 19.52),(132.61 ± 18.03) U /mg protein, group there was significant difference between(P <0.05); MDA contents were(2.45 ± 0.72),(3.31 ± 0.75),(5.11 ± 1.02),(7.47 ± 1.43) nmol / mg protein, the difference between groups was statistically significance(P <0.05).Conclusions:The use of high-dose ceftriaxone can lead to the occurrence of kidney stones; large doses can cause kidney tissues Ceftriaxone increase of MDA and SOD activity reduced,indicating that the use of high-dose ceftriaxone can cause renal tubular epithelial oxidative stress.