Study Of Alginate-based Hydrogels For Neural Stem Cells Induced Differentiation

Central nervous system injury seriously influences our human beings due to undegenerated central nervous system. Using self-renew stem cells supply a promising strategy for curing central nervous disease. However stem cell transplantation has two challenges, one is the poor survival rate, and another is limited efficient differentiation, for example, only 5% neural stem cells differentiation into neurons in vivo without any treatment. Aiming at the two challenges, we designed two hydrogels for neural stem cells transplantation to solve the problems from several aspects.Different degree of oxidized partial oxidation of sodium alginate were prepared at first, choose the best one by stiffness and micro-topography. Combine with TWS119 nanoparticles We tested the one by biocompatibility and the efficiency of effective differentiation. The result showed that 90% of stem cells were induced to differentiate into neurons, but the hydrogel can not induce to differentiate into dopaminergic neurons specially A9 subtype for Parkinson’s disease.Aiming to defect of A9 subtype dopaminergic neurons and two challenges of stem cell transplantation, we designed double layer intelligent structure hydrogel.We combined RGD peptides and MMP substrate peptides with the core layer to promote adhesion, proliferation and migration of neural stem cells. Neural stem cells secreted MMP interaction with core layer MMP substrate peptides, when acute inflammatory reaction was gone, stems cells migration to touch the outer layer.Ab Cripto-1 immobilized out layer can differentiate NSCs into dopaminergic neurons specially A9 subtype and form the functional network.