Expression Of CTGF、COL-I、PPARγ On Ovary Extracellular Matrix And Their Relationship With Polycystic Ovary Syndrome

Objective:This study adopts immunohistochemistry method to detect the expression and relationship of connective tissue growth factor, type I collagen and peroxisome proliferator-activated receptor γ in the ovarian tissue of polycystic ovary syndrome patients and the ovarian tissue of patients with oophorectomy for the purpose of discussing the relationships of CTGF, COL-I, and PPARγ on PCOS patients’ ovarian fibrosis and capsular thickening,as well as providing some clinical data for the treatment of PCOS stromal hyperplasia.Methods:1. Select 13 cases of PCOS ovarian tissue as research group; select 50 cases of ovarian tissue of patients with oophorectomy( 25 cases as reproductive age group, 25 cases as post-menopausal age group) as control group.2. Gather the clinical data of research objects, including age, history of present illness,past history, menstrual history, obstetrical history, family history, treatment through, body mass index.3. Carry out fixation, embedding, and section for selected tissues; after HE staining, carry out pathological tissue morphologic observation and analysis; apply immunohistochemistry SP method to detect the positioning expression and distribution of CTGF, COL-I, and PPARγ in the ovarian tissue of PCOS patients and the ovarian tissue of patients with oophorectomy, and apply leica QW 500 MC image analysis system to detect the strength of positive expression of CTGF, COL-I, and PPARγ in the tissue specimens; apply stereological methods(positive stained area method and integrated optical density method) to show the expression state of CTGF, COL-I and PPARγ.4. Adopt SPSS17.0 analysis software to carry out a statistical processing of experimental data. The strength of positive expression of CTGF, COL-I and PPARγ is shown via( sx ±). Among groups use analysis of variance between-group differences(one-way ANOVA), correlation analysis use pearson correlation analysis, and P﹤0.05 is used as the standard to judge whether the difference is of statistical significance.Result:1. Comparison on pathological tissue morphology after HE staining As for PCOS group, the ovarian albuginea gets thickening and fibering with multiple cystic follicles, the quantity of cells at stratum granulosum of vesicular follicle obviously decreases, and LUF doesn’t exist and even disappears; as for reproductive age group of control group, it is able to see follicle of different development stages in the ovary, there are large quantity of cells at stratum granulosum, and LUF can be seen; as for menopausal age group of control group, the ovarian tissue atrophies, the primordial and growing follicles decrease, the quantity of atresia follicle increases without typical mature follicles seen, and it is able to see irregular arrangement of granulosa cells; the quantity of degraded corpus luteum increases and the quantity of mature corpus luteum is few, with loose mesenchyme.2. Result of immunohistochemistry analysis2.1 Location and distribution: it is found that CTGF positive granule is expressed in cytoplasm and cell membrane, COL-I shows as a brown granule belt and it is distributed in ntercellular substance, and PPARγ positive granule is expressed in the nucleus.2.2 Semi-quantitative analysis2.2.1 The IOD values of CTGF expression in PCOS group, reproductive age group and menopausal age group are 47.666±9.227,15.056±5.015,17.320±3.423 respectively; the positive unit of them are 15.78±2.69、8.14±1.67、7.14±1.57 respectively. Through comparison between PCOS group and reproductive age group and menopausal age group,the difference is of statistical significance(p <0.01), but the difference between reproductive age group and menopausal age group is of no statistical significance(p>0.05).2.2.2 The IOD value of COL-I expression in PCOS group, reproductive age group and menopausal age group are 23.479±2.078,13.013±3.854,14.228±1.587 respectively; thepositive unit of them are 14.83±3.73、7.36±2.49、6.43±1.28 respectively. Through comparison between PCOS group and reproductive age group and menopausal age group,the difference is of statistical significance(p <0.05), but the difference between reproductive age group and menopausal age group is of no statistical significance(p>0.05).2.2.3 The IOD value of PPARγ expression in PCOS group, reproductive age group and menopausal age group are 3.998±0.8057,6.8014±1.39,6.8230±2.54 respectively;the positive unit of them are 5.96±0.63、13.93±1.53、12.67±1.63 respectively. Through comparison between PCOS group and reproductive age group and menopausal age group,the difference is of statistical significance(p <0.05), but the difference between reproductive age group and menopausal age group is of no statistical significance(p>0.05).3. Through statistics by use of Pearson’s product-moment correlation method, it can be obtained via analysis that there is significant correlation between increase of COL-I content and increase of CTGF expression(r = 0.692, P< 0.05), significant correlation between increase of COL-I content and decrease of PPARγ expression(r =-0.574, P<0.05), and no significant correlation between the expressions of CTGF and PPARγ(r =-0.412, P> 0.05).Conclusion1. HE staining: As for PCOS, the ovarian albuginea gets thickening and fibering with multiple cystic follicles is in line with the histological and pathological features of PCOS.2. Both CTGF and COL-I have positive expression in ovarian stroma, theca cells and granule cells of PCOS group; compared with the control group, the distribution and content of CTGF and COL-I obviously increase, thus CTGF and COL-I may be involved in PCOS ovarian stroma hyperplasia and cause PCOS ovulation disorders. PPARγ has positive expression in varian stromal and granulosa cells of PCOS group, and it is mainly distributed in granule cells; compared with the control group, the PPARγ expression of PCOS group significantly decreases, causing the increase of ovarian ECM components,thus PPARγ may be involved in the process inhibiting ovarian stromal hyperplasia.3. In correlation analysis, the expression of CTGF and COL-I shows a significant positive correlation, the expression of PPARγ and COL-I shows a significant negative correlation, which shows that the increase of CTGF expression strength and decrease of PPARγ expression strength in PCOS ovarian tissues can cause the increase of COL-I in ovary ECM and then cause the possibility of PCOS ovarian interstitial fibrosis.