Expression Of Melatonin Receptor In Fetal Skins And Hypertrophic Scars

Objectives To study the expressions of fetal skins as well as hypertrophic scars of melatonin receptor in various developing stages and to investigate the relationship between the shaping of hypertrophic scar and the expression of melatonin receptor, providing theory evidence for preventing and curing scar hypertrophic scars.Methods 24 samples were embryo gestational age(EGA) from 12 to 34 weeks of fetal skins,which divided into 3 groups in accordance with EGA which are early stage(EGA 12-19 w, 6 samples), middle stage(EGA 20-27 w, 12 samples) and late stage(EGA 28-34 w, 6 samples). 8 samples of fetal skins and hypertrophic scar tissues were obtained from patients who underwent comstic surgery. The process of scar formation were 3 to 12 months.1.Hematoxylin-eosin staining were performed to different periods of fetal skins、adult skins and hypertrophic scar tissues, observing their prostatic structure.2.Examined the expression of receptors express of fetal skins 、 adult skins and hypertrophic scar tissues in different periods by immunohistochemical staining.3.Examined the expression of mRNA of fetal skins、adult skins and hypertrophic scar tissues by quantificational rt-PCR.Results 1.MophologicalHematoxylin-eosin staining showed that skin biopsy of fetus contain epidermis, dermis and and underlying fatty layers. In fetal early development, epidermis layer contained only two to three layers of epidermal cells.Stratum spinosum of the epidermis and structure of hair follicle didn’t completely shaped. Sweat gland in dermis started to develop, fibroblasts of dermis were in fusiform shape,with dark cell nucleus and small nuclei. Volums of basal cells were small. As fetus continued to develop, numbers of fibroblasts increaseed, in the form of star, cell nucleus scaled up. Appendix organs were mature than before.Capillaries were narrow. Numbers of blood vessels increased, and developed into adult skin.2.The expression of melatonin receptor in fetal skins and hypertrophic scarsImmunohistochemistry revealed that positive expression of melatonin receptor GPR50 existing fetal skins in different stages. Late stage fetal skins were highly positive in the expression of melatonin receptor GPR50 than middle stage(P<0.05). Middle stage fetal skins were highly positive in the expression of melatonin receptor GPR50 than early stage. while early stage fetal skins had a low expression(P<0.05). Melatonin receptor was positively expressed in hypertrophic scars, normal skins and in hair follicles, epidermal layers, sweat glands and capillaries of fetal skins. The positive expression of melatonin receptor in hypertrophic scars were higher than that in adult skin tissues(P<0.05). The positive expression of melatonin receptor in adult skins were higher than that in fetal skins in middle stage(P<0.05).3.The expression of m RNA of melatonin receptor in fetal skins and hypertrophic scarsThe expression level of MT1 and MT2 m RNA of fetal skins in late stages(2.32±0.27、4.67±2.03) higher than that in middle stage(1.94±0.63,2.22±1.05).The expression level of MT1 and MT2 m RNA of fetal skins in middle stageshigher than that in early stage(1.37±0.36,1.50±0.41)(P<0.05),which increased with gestational age. MT1 and MT2 m RNA expression in hypertrophic scars(6.683+2.391,5.755+0.849)was higher than that in normal adult skins(P<0.05). MT1 m RNA expression of fetal skins in middle stage was lower than that in normal adult skins(P<0.05).Conclusion As gestational age increased, fetal skin developed too. The expression of melatonin receptor increased gradually. The creation and formation of hypertrophic scars were possibly relate to the expression of melatonin receptor...