| The volatile oils of Angelica(S-VOAS) and its different processed products including parching Angelica with wine(J-VOAS), parching Angelica with oil(O-VOAS), parching Angelica with soil(T-VOAS) and charred Angelica(C-VOAS)were used as the research object in this study. The systemic inflammation model of rat was caused by lipopolysaccharide(LPS). The rats were divided into control group,model group, angelica naphtha group(S-VOAS), wine angelica naphtha group(J-VOAS), oil angelica naphtha group treatment group(O-VOAS), udo violate oil group(T-VOAS), carbon angelica naphtha group(C-VOAS) and positive control group(Dex). The blood routine index, serum biochemical indices and related cytokines of different rats were detected and the change of liver tissues and lung tissues of rats in different groups were observed. On this basis, the metabolic fingerprint of plasma, liver and lung tissue homogenate and urine of rat were built using Gas Chromatography-Mass Spectroscopy(GC-MS) as the analysis method. The differences metabolites between control group and model group were analyzed by principal component analysis(PCA) and partial least squares-discriminate analysis(PLS-DA). The potential biomarkers were identified according to the load matrix,variable importance in the projection(VIP) and t-test and the metabolic pathways were established. The main results were as follows:1. The violate oils of Angelica processed products had the function of decreasing the amount of white blood cells(WBC) and neutrophils(NE), inhibiting the secretion of inflammatory mediator NO and pro-inflammatory factors TNF-α、IL-6、IL-lβ、IL-2,and promoting the secretion of IL-10. This indicated that the anti-inflammatory mechanism of violate oil of Angelica was closely related with inflammatory factors.In addition, all the violate oils of Angelica processed products showed good anti-inflammatory effects, in which J-VOAS showed the best effect.2. 11 kinds of potential biomarkers correlated to inflammation induced by LPS had been identified. These metabolites were mainly concerned with arachidonic acid metabolism, pyruvate metabolism, glycine, serine and threonine metabolism,tryptophan metabolism and citrate metabolism, indicating the volatile oils of different Angelica processed products play a role in immune-enhancement and anti-inflammation by cutting off the secretion of prostaglandin and mobilizing the amino acid metabolism, and maintain the stability of the body by compensatory enhancement of energy metabolism. Moreover, the protective effect on inflammation model of J-VOAS was proved to be better.3. 14 kinds of potential biomarkers correlated to inflammation induced by LPS had been identified in liver homogenate. The liver metabolic disorder in model rats were mainly related to linoleic acid metabolism, arachidonic acid metabolism,galactose metabolism, tryptophan metabolism, citrate metabolism and fatty acid metabolism. 12 kinds of potential biomarkers correlated to inflammation induced by LPS had been identified in lung homogenate. The lung metabolic disorder in model rats were mainly related to linoleic acid metabolism, D-glutamic acid and D-glutamine metabolism, alanine, glutamic acid and glutamic acid metabolism,galactose metabolism, arachidonic acid metabolism, glycine, serine and threonine metabolism and citrate metabolism. When the model rats were administrated with volatile oils of different Angelica processed products, the levels of these metabolites showed a trend to return to normal, which suggested the anti-inflammatory effect was exerted by cutting off the secretion of prostaglandin, mobilizing the amino acid metabolism and regulating the content of free fatty acid, and maintain the stability of the body by citrate metabolism and galactose metabolism. Compared with other groups, J-VOAS showed better anti-inflammatory effect.4. 12 kinds of potential biomarkers correlated to inflammation induced by LPS had been identified in urine. The urine metabolic disorder in model rats were mainly related to linoleic acid metabolism, arachidonic acid metabolism, pyruvate metabolism, D-glutamic acid and D-glutamine metabolism, citrate metabolism,glycine, serine and threonine metabolism, butyrate metabolism, glycolysis and gluconeogenesis. The inflammation in rats alleviated by being administrated with volatile oils of different Angelica processed products, which suggested the anti-inflammatory effect was exerted by cutting off the secretion of prostaglandin,mobilizing the amino acid metabolism and butyrate metabolism, and maintain the energy metabolism of the body by citrate metabolism, glycolysis and gluconeogenesis.Compared with other groups, J-VOAS showed better anti-inflammatory effect.In conclusion, the volatile oils of different Angelica processed products possibly intervened with the metabolic process of inflammation by altering lineoleic acid metabolism, arachidonic acid metabolism, citrate metabolism, glycolysis and gluconeogenesis, fatty acid metabolism, pyruvate metabolism, glycine, serine and threonine metabolism and tryptophan metabolism. The biomarkers of biological statistics and biological significance were screened and the anti-inflammatory mechanism and effect of the volatile oils of different Angelica processed products on acute inflammation were evaluated on the whole reveals. On the whole, the anti-inflammatory effect of J-VOAS is better. This study provided a feasible plan and technical support for the comprehensive evaluation of efficiency mechanism of traditional Chinese medicine(TCM).Key words: Metabolomics; Inflammation; Lipopolysaccharide; Angelica sinensis;... |
PDF Full Text Request