Study On The Expression Change Of Glutamate Cysteine Ligase Gene In Rheumatoid Arthritis

Purpose: Rheumatoid arthritis(RA) is a common chronic autoimmune disease. Its pathogenesis and pathological process has been a hot research. For the study of oxidative stress state, especially glutamate cysteine ligase(GCL) of rheumatoid arthritis disease, we should choose the appropriate method of glutamate cysteine ligase catalytic subunit(GCLc) and glutamate cysteine ligase modulatory subunit(GCLm) analyzed in rheumatoid arthritis patients, to illustrate the role of oxidative stress in the pathogenesis of rheumatoid arthritis.Methods: Early morning fasting venous blood were acquisited between rheumatoid arthritis patients and healthy controls. RF, CRP, C3, C4, Ig G, Ig A, Ig M of peripheral blood mononuclear cells were analyzed by nephelometric immunoassay Analyzer, ESR was detected by using instrumental method and anti-CCP antibodyies were assayed through ELISA. The peripheral blood lymphocytes RNA were separated by density gradient centrifugation using the Ficoll Hypaque system and RNA by reverse transcriptase. The expression levels of glutamate cysteine ligase catalytic subunit and modulatory subunit were analysed by real time fluorescence quantitative PCR which was verified amplification of specific by solubility curve.Results: Laboratory data of rheumatoid arthritis patients group supported that the value of anti-CCP, RF, CRP, Ig G and ESR increased while the value of C4 decreased. Compared with healthy controls, the expression levels of glutamate cysteine ligase catalytic subunit and modulatory subunit in peripheral blood mononuclear cells from rheumatoid arthritis patients obviously decreased(GCLc:RA 1±0.018, Healthy controls 3.62±0.429;p<0.01.GCLm:RA 1±0.03, Healthy controls 2.97±0.467;p<0.01).Conclusions: The obvious changes of glutamate cysteine ligase catalytic subunit and modulatory subunit during the disease course of rheumatoid arthritis and the significantly decreasing expression levels indicated that the oxidative stress state and glutathione metabolism may play an important role in the process of rheumatoid arthritis disease, and further studies should be directed to the pathogenesis of rheumatoid arthritis.