Expression Of Protein In Nude Mouse Model Bearing Orthotopically Transplanted Human Ovarian Carcinoma Induced By Adverse Psychological Stress

Bbackground:Ovarian cancer is a common gynecologic malignant tumor. Due to the incidence of occult, early diagnosis is very diffcult, most of patientas were present with disease that has distant metastasis on the top of the case fatality rate of gynecological malignant tumor. Although ovarian cancer treatments, such as surgery, radiotherapy and chemotherapy treatment methods and so on, continue to ameliorate in recent years, but the treatment effect is not ideal and mortality rates did not decline in nearly three decades. Survival rate of 5 years of Ovarian cancer hovers around 30% to 40%. By the above it is not hard to see ovarian cancer threat seriouly women’s quality of life and the life and health. With modern medical model convert to the biological-psychological- social pattern transformation, human gradually found that tumor is influenced by many factors as regards the occurrence and development of tumor. It has been established that the chronic psychological social factors accelerate the occurrence and development of multiple tumor, and the tumor regarded as a psychosomatic disease gradually. Therefore, Those have increasingly become the key point of medical research institute, further research the relation between psychological and tumor and its impact mechanism, using psychological therapy to control the occurrence and development of tumor, or by changing the tumor patients to control tumor immune status.Purpose: 1、The establishment of the chronic psychological stress models on nude mouse bearing orthotopically transplanted human ovarian cancer.2 、 To explore the expression of protein in nude mouse model bearing orthotopically transplanted human ovarian carcinoma induced by adverse psychological stress and to discuss the molecular mechanisms of the chronic psychological stress affect ovarian cancer.Research Methods: 1、The classification of nude mouse and to build chronic psychological stress model in nude mice.2、The establishment of nude mice model bearing subcutaneous transplantation human ovarian cancer.3、The structureer of nude mice model bearing orthotopically transplanted human ovarian cancer.4、The extraction of orthotopic transplantation tumor tissue samples of protein.5、 The Western blotting of protein sample and the comparison of protein expression(Prdx1,SOD1,GSTO1,Nm23,CK) between stress group and matched group.6、The Immunohistochemical of orthotopic transplantation tumor tissue and the verification of protein expression(Prdx1,SOD1,GSTO1,Nm23,CK) between stress group and matched group.Results: 1、Nude mice survival and weight About 3 days before the experiment, the strong stress group of nude mice is nervous, agitated,and adaptive a few days latter. Match with the control group, the stress group of nude mice have poor growth state,limited food intake, less desire and range of activity decreased, slow reaction, slow weight gain,same water intake yet, without die nude mice in the experiment. After the test, the nude mice weight of the stress group was obviously lower than the control group, The difference was significant(P < 0.05), and before the test, there was no statistically significant difference between groups(P > 0.05).2、tumor weight About 4 weeks after bondage stress, we can touch 0hard nodule in nude mice abdomen, over time, the mass increase gradually, the abdominal circumference increase gradually, the subcutaneous fat loss gradually. In general view, the stomach neoplasm of the stress group of nude mouse growth faster than the control group.Death are did not happen, and the tumor rate of Orthotropic transplantation tumor surgery is 100% on the whole test process. After the test, carefully peeling the tumor and weighing, the tumor weight of the stress group of nude mouse was obviously higher than the control group, there was no statistically significant difference(P < 0.05).3、 the Western blotting of 5 proteins in the nude mouse orthotropic transplantation tumor tissueBy the Western know, the tested four kinds of proteins, including the Prdx1, SOD1, GSTO1, Nm23, expression in stress group was obviously higher than the control group of nude mouse orthotropic transplantation tumor tissue,but the CKM protein expression was lower, the difference between groups were statistically significant(P < 0.05).4、Immunohistochemical results of human ovarian cancer in situ tumorburdened nude mice transplantation tumor tissueThe nude mouse orthotropic transplantation tumor tissue, between the stress group and the control group, after HE staining confirmed by microscopy is poorly differentiated adenocarcinoma, and conform to the pathological features of ovarian cancer.It showed by microscopy after immunohistochemical staining. Compared with the control group, the protein positive rate of the Prdx1, SOD1, GSTO1 and Nm23 in the stress group is significantly higher than the control group, however the CKM protein positive rate is lower, the differences were statistically significant(P < 0.05).Conclusion: This experiment successfully built the chronic psychological stress model in nude mouse bearing human ovarian cancer orthotropic transplantation tumor, and further confirmed what exposed to chronic psychological stress can cause tumor protein happen change on quantity and quality of protein. These proteins may be involved in the regulation of cellular energy metabolism, the formation of the cytoskeleton and the process of cellular proliferation, differentiation, apoptosis, etc, thus to influence tumor occurrence, development and outcome. This experiment provides a new theoretical basis for molecular biology by to reveal the relationship between psychology and tumor.