The Research Of The Parts Of Cell Proliferation Effect Of Velvet Antler Its Principal Component Analysis

The antler is a bone tissue which can be regenerated postnatally annually. We earn this kind of relatively benign medicines from Cervus nippon Temminck and Cervus elaphus Linnaeus. Its main effect include the problem of male and female reproductive, at the same time it also have effect on ear pathema.In this study, the method of DPPH, ABTS and reducing activity assay were used to determine the extraction’s antioxidant activity in vitro of the Reindeer antler and Sika Deer Antler. The data indicate that both extractions of them have the antioxidant activity. The water extraction is better than the alcohol extraction. There is a positive correlation between antioxidant capacity and concentration. The result of the assay provide a basis for further development of antler. It means that the antler as a natural antioxidant its mechanism of antioxidant activity need for further pharmacology research.Use ether, chloroform, ethyl acetate, ethanol, water as solvent extract the different part of the Reindeer antler and determine the effect on proliferation of human renal fibroblasts. The water extraction had the extremely significant from day3-9. When the concentration in 10μg/m L,100μg/m L it had obvious effect(P<0.01) with the rate 116.5%,172%; at the fifth day it has the highest effect with the rate 276.3%. The ethanol extract in the concentration 1mg/m L had proliferation rate of 31% at the 7th day. The data above show that the effect of Reindeer antler invigorate and strengthen the kidney is accomplished by improve the activity of human renal fibroblasts’ proliferation.With the method of MTT and LDH, determine the protective effect of Reindeer antler(ether, chloroform, ethyl acetate, ethanol, water) on human embryonic kidney(HEK293) cell injury model. Establish the model by CDDP. The data indicate that the extract of Reindeer antler had the rivalry to the model and there is a positive correlation between ability and concentration, and the IC50 is 0.083±0.03 mmol L-1. At the concentration in 1mg/m L water extraction had the strongest protective effect with the rate 62%(P<0.01), and compared with the others water extraction has the best effective on the model.With the method of MTT and LDH, use the cell apoptosis rate as the index, determine the protective effect of Sika Deer antler on human embryonic kidney(HEK293) cell injury model. At the same time use the SOD,MDA kit determine the influence on(HEK293) cell injury model. The data indicate that with the increase of the concentration of protein and polysaccharide of Sika Deer antler, cell injury rate reduced. Determine with the SOD,MDA kit, the extraction of Sika Deer antler can increased the superoxide dismutase content and reduce the content of MDA. The protein had the best effect on the model and may be associated with MDA, SOD content.The principal component analysis method was used to compare the difference between different antler. The data indicate that Factor 1 and Factor 2 had the largest difference of characteristic value. With the variance contribution rate 57.128% and 21.59%. Factor 1, the cumulative contribution rate was 57.128%, less than 70%, the cumulative contribution rate of Factors 2 was 78.718%, the cumulative contribution rate of Factors 3 was 93.792%. The characteristic components of antler are antler protein, moisture and the water extract, but not the other components. Compare the similarities and differences of 9 nutritional composition from different antler, provide a theoretical basis for the study on the quality evaluation of antler.