The Effects Of Aspirin On EMT Of Human Colon Cancer Cell Line HT-29

Objective: To investigate the effects of non-steroidal anti-inflammatory drugs aspirin on epithelial-mesenchymal transition of cultured human colon cancer cell line HT-29 and possible mechanism.Methods: 1.The effect of different concentrations of aspirin on the growth and proliferation of HT-29 cells was tested by MTT;2.After treatment with aspirin,Transwell chamber method and wound assay was used to estimated the invasion and migration of HT-29 cells respectively;3.Western blot and Real-time quantitative PCR was used to detect the protein and m RNA expression of E-cadherin、N-cadherin、Vimentin and Snail1 of EMT important markers in HT-29 cells.Results: 1.MTT assay showed that the effect of aspirin on the growth and proliferation of HT-29 cells has a dose and time-dependent increase inhibition;2.Aspirin has a significant inhibitory effects on the migration and invasion of HT-29 cells(P<0.05,P<0.01);3.real-time PCR and Western blot have revealed that after the treatment with aspirin,the m RNA and protein expression of E-cadherin was higher than the control group(P<0.05),and N-cadherin、Vimentin and Snail1 in HT-29 cells were lower than the control group(P<0.05).Conclusion:1.Non-steroidal anti-inflammatory drug aspirin can inhibit the growth and proliferation of human colon cancer cell line HT-29; 2.Aspirin can inhibit the migration and invasion of HT-29 cells; 3.After treatment with aspirin on HT-29 cells,the expression of E-cadherin was increased,the expression of N-cadherin、Vimentin and Snail1 were declined,the results showed that aspirin can inhibit the EMT process.