The Correlation Between Serum TNF-α With The Severity Of Condition In Acute Pancreatitis

Objective: To discuss the relationship between tumor necrosis factor alpha(TNF-α) with the condition and prognosis of acute pancreatitis by detecting the levels of serum TNF-α in acute pancreatitis patients. Methods: Collected Patients with acute pancreatitis which treated in Qinghai Province People’s Hospital from December 2013 to October 2014. There were 20 patients with severe acute pancreatitis and 20 patients with mild acute pancreatitis. All the patients got to hospital in one day and had not received treatment. There were 12 health volunteers which participated in medical examination in Qinghai People’s Hospital as control group. The AP group’s venous blood were collected at the 1st day, 3rd day, 7th day and 14 th day, and the control group’s venous blood were collected in the 1st day when they were examined. The levels of serum TNF-α were measured by using ELISA method. Analyzed the changes of serum TNF-α level in the different time. Did the correlation analysis between the levels of TNF-α with APACHE Ⅱ score in severe acute pancreatitis.Results: The levels of TNF-α on 1st day in the MAP and SAP group compared with control group were significant higher(P<0.05), and the levels of TNF-α in SAP group compared with MAP group were significant higher(P<0.05). After treatment, the levels of TNF-α on 3rd day in the MAP group began to decrease, but compared with the control group were still significant higher(P<0.05); the levels of TNF-α on the 7th day and 14 th day in MAP group got close to the control group, and compared with the control group were no significant difference(P>0.05); the levels of TNF-α on 3rd day in SAP group compared with the 1st day were significant higher(P<0.05); the levels of TNF-α on the 7th and 14 th day began to decrease(P<0.05), but compared with the MAP and the control group were significant higher(P<0.05); The levels of TNF-α were positively correlated with the APACHE Ⅱ scores in the SAP group(r=0.88, P<0.05). Conclusions: ①The TNF-α play an important role in inflammation reaction of acute pancreatitis, and it can be used as a clinical assessment on the severity of acute pancreatitis. ②The TNF-α can be helpful to predict the outcome of AP patients.