The Effect Of The DMSCs Of Psoriatic Patients Lesions On Ha-cat Cell

Objective:To establish DMSCs( human dermis mesenchymal stem cells)culture system through DMSCs of psoriatic patients isolated,cultivated and co-culture with Ha-cat in vitro,and to study the effect of DMSCs on Ha-cat Cell in Patients with Psoriasis Lesions. Methods:1.The mononuclear cells were isolated in psoriatic patients lesions and in healthy controls skin respectively;2.The cell surface markers of CD and HLA-DR were detected by FCM;3.The multi-directional differentiation cells were identified by induced cells through corresponding inducers;4.The proliferation of Ha-cat cell was investigated in a co-culture system by real-time cell analysis system;5.The proliferation of Ha-cat cell was investigated in a co-culture system by cell counting,which after 74 hour;6.Cell cycle determination of Ha-cat cells in DMSCs co-culture by flow cytometry. Results:1.Two group of DMSCs were observed under inverted microscope;2.FCM showed that the DMSCs surface markers CD(29、44、105、73) and CD90 were highly expressed,and CD(34、45)and HLA-DR were expressed lower,which proved that cultivate DMSCs have higher purity;3.DMSCs can be differentiated to adipose cell,osteoblast and gristle in extracorporeal derivant.4.Ha-cat cell proliferation activity test in a co-culture system by real-time cell analysis system:DMSCs in psoriatic patients lesions has advance effect on Ha-cat cell proliferation5.The result of Ha-cat cell proliferation activity test in a co-culture system by cell counting:DMSCs has advance effect on Ha-cat cell proliferation in psoriatic patients lesions(P<0.05).6.The result of cell cycle determination of Ha-cat cells by flow cytometry: The percentage of S phase for Ha-cat cells co-cultured with psoriatic DMSCs is significantly higher compared with co-cultured with control DMSCs(P<0.05). Conclusions:1.Establish the cultivation system of skin DMSCs with Ha-cat in vitro;2.DMSCs derived from psoriatic skin lesions promote the proliferation of Ha-cat Cells.