Research On Relations Between Polymorphism Of HLA-DQ Gene Rs9275319 And Hepatitis B-related Liver Cancer

Objectives:1. Detection of locus rs9275319 of HLA-DQ gene and analysis of its effect on hepatitis B-related liver cancer. 2. Discussion of relevance of polymorphism of locus rs9275319 of HLA-DQ gene and polymorphism of upstream HLA-DQB1 gene to occurrence of hepatitis B-related liver cancer.Methods:1. 363 cases of hepatitis B-related liver cancer patients and their family members in Shanxi were chose as objects of study, among which there were 132 cases in the hepatic cellular cancer(HCC) group, 81 cases in the hepatitis B-liver cirrhosis(HC) group, 100 cases in the chronic hepatitis B(CHB) group and 50 cases in the normal control group; according to research objects’ conditions of their family history, groups were further subdivided into groups with and without a family history. PCR-direct sequencing was applied to test gene polymorphism of locus rs9275319 of HLA-DQ gene of familial hepatitis B patients and their family members. Chi-square tests were used to compare experimental results among groups and discuss relations between locus rs9275319 and hepatitis B-related liver cancer of Shanxi. 2. 114 hepatitis B-related liver cancer patients were taken as subjects of study and 50 healthy persons from their families were chosen as normal control. PCR-direct sequencing and polymerase chain reaction-sequence based typing(PCR-SBT) were applied to test polymorphism of locus rs9275319 of HLA-DQ gene and polymorphism of upstream HLA-DQB1 gene respectively and statistical analysis was carried out on experimental results by association tests of bothway unordered data. Whether they had cooperativity on effects of occurrence of hepatitis B-related liver cancer was discussed.Results:1. After genetic typing of rs9275319 of HLA-DQ gene, the frequency of allele A was significantly higher than that of allele G. By comparison among groups, it was found out that the homozygote AA genotype frequency(91.7%) in the hepatic cellular cancer(HCC) group was higher than that of the CON group(76%). Their differences were of statistical significance(P﹤0.05) and the OR value was 3.865. 2. The homozygote AA genotype frequency(97.9%) in the hepatic cellular cancer(HCC) group with family history of hepatitis B was higher than that of the CON group(76%). Their differences were of statistical significance(P﹤0.05) and the OR value was 4.375. 3. HLA-DQB1 *0202 and *0301 genotype frequencies(12.7% and 30.2%) of the liver cancer group were higher than those(5% and 18%) of the normal group respectively. Their differences were of statistical significance(their X2 were 4.44 and 5.31 respectively, 0.01<P<0.05). 4. HLA-DQB1 *0202 and *0301 genotype frequencies(15.4% and 32.1%) of the HCC group with family history of hepatitis B were higher than those(6% and 19%) of the normal group respectively. Their differences were of statistical significance(their X2 were 4.246 and 4.011 respectively, P<0.05). 5. It was possible that polymorphism of locus rs9275319 of HLA-DQ gene and upstream HLA-DQB1*0202 and *0301 gene was not relevant with effects of occurrence of hepatitis B-related liver cancer.Conclusions:1. It was possible that polymorphism of locus rs9275319 of HLA-DQ gene was a risk gene of hepatitis B-related liver cancer and familial hepatitis B primary liver cancer. 2. It was possible that HLA-DQB1*0202 and *0301 alleles were susceptibility genes of hepatitis B-related liver cancer and familial hepatitis B primary liver cancer. 3. It was possible that polymorphism of locus rs9275319 of HLA-DQ gene and upstream HLA-DQB1*0202 and *0301 gene was not relevant with effects of occurrence of hepatitis B-related liver cancer.