Effect Of Artemisine On Proliferation And Apoptosis Of Human Colon Cancer HCT116 And HT29

Objective:To study the effects of artemisine on proliferation and apoptosis of human colon cancer HCT116 and HT29 cells.For provide new adjuvant therapy for clinical human colon cancer treatment.Methods:Human colon cancer cell treatment with different concentrations determined by MTT artemisine assay was used to detect cell proliferation and FCM to detect cell apoptosis. The Application SPSS13.0 statistical software for processing experimental date are expressed as mean ±standard deviation(X±s), using a one-way analysis of variance and comparing two group, betwent group used independent samples t-test analysis.Results:The inhibition rates had upwarded trend with increasing concentrations(HCT116 :19.42%、24.59%、44.84%、74.15%、92.87%;HT29:11.77%、 17.63%、34.23%、48.51%、70.59%),when treated with artemisine at difierent concentrations( 7.5、15、30、60、120μg·ml-1)for 48 hours.And under the same concentration of HCT116 obviously stronger than HT29.Apoptosis rates gradually increased with increasing concentration(HCT116:2.46% 51.36% 64.10%;HT29:1.14% 35.95% 43.45%),when treated with artemisine at different concentrations(0、60、120μg.ml-1)for 24 hours.Conclusions:Artemisine inhibits proliferation and induces apoptosis of human colon cancer cells HCT116 and HT29.And under the same concentration of HCT116 obviously stronger than HT29.