Study On The Diagnostic Value Of Detecting Sensitized Umbilical Cord Blood Erythrocyte In Hemolytic Disease Of The Newborn

Objective:By using flow cytometry, the positive cells which were labeled with FITC-Ig G in cord blood was detected. The positive cells were the fetal red blood cells sensitized by the maternal blood group antibody.In order to explore the clinical diagnostic value of this method of ABO hemolytic disease of the newborn.Methods:1.ABO blood type and RH blood testing, the surface blood group phenotypes of neonatal red blood cells were examined by using the ABO,Rh D Blood Grouping Cards in order to screen newborns whose ABO blood group were incompatible with his mother.2.Transcutaneous bilirubin(TCB),by using the TCB instrument, TCB of the newborns was dynamically monitored everyday and the results were recorded within 3days after birth.3.The testing of the positive cells labeled with FITC-Ig G,we collected umbilical cord blood 2ml,whose mother blood type was O, collected peripheral venous blood4 ml,who was diagnosed of hemolytic disease of the newborn, collected peripheral venous blood 2ml, who was due to other diseases, placed them in EDTA vacuum tubes, mixed and preserved them under the 4 degree centigrade.American BD Company provided the BD FACSCalibur flow cytometer.UK Abcam Company provided the goat F(ab ’) 2 Antihuman Ig G H & L(FITC). Fluorescence labeling and flow cytometry were used to detect the percentage of sensitized red cells. Operation was strictly according to the instructions.4.Hemolytic series, the peripheral venous blood(2ml)of the children who were suspected of hemolytic disease of the newborn was sent to blood center and detected threehemolysis test.5.Statistical analysis, the experiment research used SPSS22.0 statistical software to deal with the data,Conform to the normal distribution of quantitative data statistical description.Quantitative data which was in accordance with normal distribution was measured with mean standard deviation said,inter group was compared with t test, the test level of α =0.05. Inter group comparison of qualitative data set was used the chi-square test,the test level of α =0.05. P<0.05 had significant difference.Results:1.Negative result threshold, the blood of the negative threshold group samples was detected by flow cytometry and the results were statistically analyzed, the percentage of positive cells in the mean was 4.719%, standard deviation was 1.641%, the negative threshold was 6.630%. If the percentage of positive cells was greater than 6.630%, the result for flow cytometry was positive, that is, Considering the occurrence of neonatal ABO hemolytic, if not, it was negative.2.Comparison of the flow cytometry test results of umbilical cord blood and peripheral blood(case group),the result of positive rate was consistent, the percentage levels of the positive cells respectively were 29.25+15.43%, 25.06+14.91%.There was not significant difference between the two kinds of blood(t=1.221,p=0.226).3.In the case group, the peripheral venous was detected by flow cytometry and three hemolysis test respectively. The results shows that in the 40 samples, the number of positive samples was 33 and negative one was 7 detected by flow cytometric, while the number of positive samples was 28 and negative one was 12 detected by three hemolysis test. The positive results of two detection probability were 82.5%,70%,the difference was statistically significant(2c =15.964,P<0.001).4.In the case group,the cord blood samples were detected by flow cytometry,while the peripheral venous samples were detected by three hemolysis test. the results shows that in the 40 samples, the number of positive samples was 33 and negative one was 7 detectedby the flow cytometric;the number of positive samples was 28 and negative one was 12 detected by three hemolysis test. The positive results of two detection probability were82.5%,70%,the difference was statistically significant(2c =15.964,P<0.001).Conclusions:1.The clinical value of flow cytometry. Compared with the three test hemolytic test,the sensitivity of flow cytometry detection is high, it can be used as one detection method when a newborn is highly suspected of ABO hemolytic disease, but three hemolysis test is negative;2.The clinical value of umbilical cord blood.The umbilical cord blood and peripheral blood were detected by flow cytometry. Their test results are unanimous; it suggests that umbilical cord blood may be one of the blood samples in diagnosis of hemolytic disease of newborn.