Influence Of Estradiol Supplementation On Pregnancy Outcomes During The Luteal Phase In IVF-ET Cycles

Objective:To evaluate the influence of estradiol supplementation on pregnancy outcomes during the luteal phase in IVF-ET cycles. Methods:In the 21 days menstrual cycle,subcutaneous injection of gonadotropin-releasing hormone agonist drop to adjust,menstrual period 2 to 7 days to drop hormone regulates level,using gene recombinant follicle stimulating growth hormone and human menopausal gonadotropin stimulate ovulation,vaginal ultrasound monitoring follicle development,when follicular diameter of 18 mm,on the evening,inject human chorionic gonadotropin 10000 iu,after34-36 hours,pick-up oocytes,after pick-up oocytes,intramuscular progesterone injection 60 mg per day,the third day transplantation,14-21 days after the transplant to check the blood and urine HCG, 25-30 days B Ultrasound determine whether clinical pregnancy.449 IVF-ET cycles with long protocol of controlled ovarian hyperstimulation(COH) were retrospectively analyzed.The patients were divided into 3 groups according to the down-range of E2 level on ET day,group A: 29 cycles,which the down-range of E2 was ≤30%, received only progesterone as the luteal phase support;group B: 186 cycles,which the down-range of E2 was 30%-50%,control group 113 cycles recived only progesterone as the luteal phase support,experience group 73 cycles received the same dosage of progesterone combined with oral E2 2mg/d as the luteal phase support;group C:234 cycles,which the down-range of E2 was ≥50%,control group 124 cycles recived only progesterone as the luteal phase support,experience group 110 cycles received the same dosage of progesterone combined with oral E2 4mg/d as the luteal phase support. Results:There were no differences on average age,duration of infertility, the number of retrieved oocytes,fertilization rates,cleavage rates and the number of transfer embryos(P>0.05); the clinical pregnancy rate were significant differences in statistic between the group A,control group B and control group C(P<0.05);The clinical pregnancy rate of estradiol valerate supplementation group was significantly higher than that in the control group(P<0.05). Conclusions:In IVF cycles,the rapidly decline of serum estradiol has the effection on the pregnancy outcome.Therefor,the proper estradiol valerate supplementation during the luteal phase may improve the clinical pregnancy rate.