Expression And Significance Of Nerve Growth Factor (NGF) In Ulcerative Colitis

Objective:To discuss the expression and significance nerve growth factor(NGF) in intestinal tissue and serum of ulcerative colitis(UC).Materials and methods:All 40 cases of patients which were diagnosed with UC from the digestive department of outpatient and inpatient in the first hospital of shanxi medical university,at the same time selected 10 patients with colon polyps and 10 cases healthy check-up as control groups,take intestinal mucosa and venous blood as the specimen. Immunohistochemical staining and ELISA methods were used respectively to detect the expression in intestinal mucosa tissue and serum of NGF in UC patients and the control groups.The results:1.Test results of immune histochemical method :The expression of NGF in UC mucosa was higher than in the control group, the grey value respectively(167.035±12.216, 188.713 ± 5.924),the difference was statistically significant(P<0.01);the expressions of NGF were differences between mild and moderate, and severe UC(P<0.05); the expression of NGF was no differences between mild and severe UC(P>0.05); NGF expression in intestinal mucosal tissue of UC was positively correlated with the degree of UC(r=0.505,P<0.05);NGF expression characteristics in the mucosa of UC unrelated to the position of the disease.2.Test results of enzyme-linked immunosorbent assay method: expression ofNGF in serum of the UC group and the healthy control group was not statistically significant(P>0.05); the concentration value of NGF expression in the serum of UC patients with the grayscale values of NGF expression in intestinal mucosa of UC patients was positively correlation(r = 0.529, P<0.05),due to grayscale values reflect to positive expression intensity of immune histochemical method,NGF expression in intestinal mucosal tissue of UC was positively correlated with the degree of UC,so the expression of NGF in serum of the UC paptients have relationship with degree of UC.Conclusions:The high expression of NGF in UC mucosa may be related to the occurrence and development of UC, and is positively correlated with the extent of the disease;NGF may be a bridge to connect the nervous system and the immune system and the endocrine system; NGF expression in the serum of patients with UC may help determine the extent of disease.