The Expression And Significance Of IL-1β、IL-6 And TNF-α In The Lesions Of Spinal Tuberculosis

Objective:To confirm the tuberculosis of spine intervertebral disc lesions of the interleukin-1β(IL-1β),the interleukin--6(IL-6)and tumor necrosis factor-a(TNF-α)factor expression and significance.Methods:WE had collected the clinical data of 25 cases(male 15,female 10)in the hospital(309hospital) from March,2013 to Match,2015.These patients ate aged 20-64 with avetage age38.3±10.57.15 cases(male 8,female 7)wete included in the studu.These patients are aged20-64 with average age 38.12±13.03.After confirmaed diagnoses,according to the imaging data before operation,observed infor information in operation and patholo gical examination after operation,there were 15 cases of vertebral burst racture,decompression and instrumented fusion,Intervertebral disc tissues were excised,which was acted as a control group.Disc material of two groups were cut respectively,then a stain for acid fast bacilli and HE stain,as well as IL-1β 、 IL-6 and TNF-α immunohistochemical stain are done.Positive expression of L-1β、IL-6 and TNF-α in two groups were observed under light microscope and positive guanules were counted.Results:With HE satain,glioma is yellow shaped like cheese with fine texture in spinal tuberculosis infection.Microscopically,it is characterized by non-guanulated structure for eosin Fibrous tissue is based on caseous necrosis by acid-fase stain.A large of inflammatory granuloma can be seen.There are positive bacillus.Mycobacterium tuberculosis ischaractered by long thin bright red bacillus arrayed in slender bands,which is clear.Other components mainly present as bright red and bright bule that is agood contrast.Immunohistochemical stain suggests that three factors IL-1β、IL-6 and TNF-α of the disc materia could be detected in two groups of patients. Upon t testing, the expression ingroups IL-1β 、 IL-6 and TNF-α of spinal tuberculosis was much higher than the normalcontrol group.There was statistically significant difference between the two groups(P<0.01).Conclusions:1.spinal tuberculosis intervertebral disc proinflammatory cytokine of IL-1, IL-6 and TNF- a large increase;2.High expression of IL-1β、IL-6 及 TNF-α in the Lesions tuberculosis mignt be good protective inflammatory response factor.、及3. Cytokines IL-1β、IL-6 及 TNF-αthat participate in the immune process might be the reason for increasing intervertebral disc erosion...