Morphological Study Of Tensile Experiment About Degenerative Nucleus Pulposus Cells

Objective:To discuss the effect on nucleus pulposus cells morphology about the tensile experiment by using the degenerative nucleus pulposus cells.To provide the oretical basis for the traction treatment of lumbar disc prolapse.Methods:The nucleus pulposus specimens were collected from patients with intervertebral disc protrusion, who were all volunteers.Nucleus pulposus cells were inoculated the cultured into the Bio Flex culture plate.The morphological changes of the cells were observed at high magnification after stretched for twenty-four hours with the tensile range of 5%、10% and 15%;The patient was given the lumber traction by using 20%、30% and35% of his body weight and the patient,s lumbar lateral radiographs were shot before and after traction.The changes of intervertebral space height were measured and calculated.Results:For the morphology of the degenerative nucleus pulposus cells,it improved mildly under the tensile range of 5%,it was close to normal nucleus pulposus cells under the tensile range of 10% and it deteriorated under the tensile range of 15% after twenty-four hours.There was significant difference between the number of cells(P<0.05).The number of cells showed that 10% tensile range group>5% tensile range group>the control group>15% tensile range group.Compared with the increasing range of intervertebral space before and after traction,there was significant difference(P<0.05).The increasing range of intervertebral space showed that 20% weight group<30% weight group<35% weight group.Conclusion:The restoration of degenerative nucleus pulposus cells was slow by using the tensile range of 5%.It was no good for the restoration of degenerated nucleus pulposus cells by using the tensile range of 15%.The restoration of degenerative nucleus pulposus cells was fast by using the tensile range of 10%.It was suitable for the patients with lumbar disc herniation by using the weight of 30% of their body weight.