FGFR3 Silencing By SiRNA Inhibits A549 Cell Invasion

Objective In order to explore the human fibroblast growth factor 3(FGFR3) gene silencing impact on the invasive ability of human lung adenocarcinoma A549 cells and matrix metalloproteinase-9(MMP9) gene expression.Methods A549 cells were classified into three groups: A: the experimental group, si RNA inhibited FGFR3 specifically si RNA-FGFR3 interference group; group B: negative control group, si RNA-NC interference group C: blank control group, no si RNA interference group. A549 cells were transfected with nucleic acid transfection reagent liposomes Lipofectamine TM2000(Lipo2000).The transfection efficiency was observed under the inverted fluorescence microscope. The invasive ability of A549 cells was detected by the transwell assay after transfection. The expression of FGFR3 and MMP9 m RNA before and after transfection were measured by Real-time polymerase chain reaction(Real-time PCR). The expression of FGFR3 and MMP9 protein before and after transfection were measured by Western blot.Results The transfection efficiency with Lipo2000 mediated FAM-si RNA to A549 cells was up to 80% under the inverted fluorescence microscope. Transwell experiments showed the invasive capability inhibited in A group than in B and C group after 36 h transfection significantly(P <0.01).Real-time PCR showed that the expression of FGFR3 and MMP9 were decreased in A group compared with B and C group significantly(P<0.01).Western blot showed that the expression of FGFR3 and MMP9 were decreased in A group compared with B and C group significantly(P<0.01).Conclusion Experimental findings indicated that FGFR3 gene silencing can inhibit the invasion ability of A549 cells and down-regulate the expression of MMP9 significantly.A new target for the treatment of lung carcinoma is supplied by this work.