Expression Of BNP In A Rat Model Of Stress Cardiomyopathy And Forensic Medical Analysis

Objective To investigate the expression of brain natriuretic peptide(BNP) protein and BNP m RNA in rat myocardial tissue in a rat model of stress cardiomyopathy and explore the role of BNP in SCD result from stress cardiomyopathy.Methods Rat models of stress cardiomyopathy were established.The expression of BNP protein and BNP m RNA in myocardial tissue after stress cardiomyopathy were detected by immtmohistochemistry and RT-PCR.Results 1.The serum cortisol concentrations in the model group(58.317±9.688nmol/L)is higher than the control group(29.712 ± 4.093nmol/L)(P<0.05).2.The serum BNP concentrations in the model group(95.088 ± 9.925ug/L)is obviously higher than the control group(60.825 ± 7.829ug/L),there were statistical differences between the two groups(P<0.05).Immunohistochemistry shows that positive staining of BNP in myocardial cytoplasm.According to semiquantitative analysis,it shows that the expression of BNP protein(0.169 ± 0.279) is higher in models than in the control group(0.144 ±0.572)(P<0.05).The expression of BNP m RNA of model group(-2.054 ±0.909)increased,there were statistical differences too(P<0.001).Conclusion In stress cardionmyopatgy disease model,myocardial tissue protein of BNP and BNP m RNA were upregulated.This factor may be involved in the acute stress leads to a complex process of sudden cardiac death.Investigating the expression of BNP protein and BNP m RNA in myocardial tissue may provide a new century to evaluate the sudden cardiac death resulting from stress cardiomyopathy for forensic pathologists.