The Influence Of MiR-27a On The Migration And Invasion Of Prostate Cancer Cells

Prostate cancer(PCa) is one of the most frequently diagnosed cancer in men worldwide, especially in more developed countries. In China, prostate cancer is the most common disease of the urinary system. The increasing incidence rate of prostate cancer in our country is due to the growth of people’s living standard and the popularization of diagnostic techniques. Because of the high mortality in metastatic prostate cancer and the reasons are still unclear, thus it is essential to better understand the underlying molecular mechanisms of the occurrence and metastasis of prostate cancer.mi RNAs are a class of small non-coding RNA as a recent hot field. And as research continues, mi RNAs play key roles in many biological processes of tumors through regulating on the target genes. mi R-27 a, as a member of them, is abnormally upregulated in breast cancer and has been identified as an oncogene in facilitating the metastasis of cancer cells. Moreover, the expression of mi R-27 a is also enhanced in prostate cancer. Thus the expression of mi R-27 a is related with the metastasis of prostate cancer.Proteasome inhibitor is a kind of anti-cancer drug, which is frequently-used during the cancer therapy. It can play its part by inhibiting the proteasome. In this study, we confirmed that the expression of endogenous mi R-27 a is positively related with the metastatic ability of tumor cells by using the real-time PCR measuring the expression of mi R-27 a in five kinds of prostate cancer cell lines. Furthermore, we examined the effect of mi R-27 a on the migration and invasiveness of prostate cancer cells. The results shown that mi R-27 a can enhance cell migration and invasion. And then, it is essential to identify mi R-27a’s target gene which can reveal the underlying molecular mechanisms. By employing the open access software, MARCKS, which can regulate the motility of cells, was chosen as a preferred candidate target gene of mi R-27 a. Meanwhile, we confirmed that celastrol can downregulated the expression of mi R-27 a through using high-throughout screening technique. That result further proved celastrol can inhibit the metastasis of the prostate caner.This study confirmed that mi R-27 a can facilitate the migration and invasiveness of prostate cancer cells. And MARCKS was screened as a novel target gene of mi R-27 a from thousands of candidates.