Studies On The Extraction Procedure Of Effective Parts From Two Kinds Of Traditional Chinese Medicine

ObjectiveIn order to make better use of Cortex Lycii and Fructus Gardeniae, optimize the best extraction technology by water-soluble total alkaloid in Cortex Lycii and gardenia yellow pigment in Fructus Gardeniae. A preliminary study to hypoglycemic of water-soluble alkaloids fraction from Cortex Lycii was carried out.MethodsThrough the single factor investigation of ethanol concentration, extraction temperature, extraction time and the ratio of material to solvent, the best extraction process of Cortex Lycii water-soluble total alkaloids fraction was optimized by response surface design method, and the verification experiments; The best extraction conditions and process of gardenia yellow pigment method from Fructus Gardeniae were optimized by orthogonal experimental design method, and the verification experiments. The lowering postprandial blood sugar in mice of water-soluble alkaloids fraction from Cortex Lycii had been investigated by intraperitoneal injection of alloxan induced diabetic mice model in mice, with acarbose positive for drugs.Results1 The best extraction process of water-soluble alkaloids fraction in Cortex Lycii are concentration of 55.4%, extraction temperature of 56.7 ℃, solid-liquid ratio of16.5 m L/g, extracting time of 180 min, and extract twice by response surface design method.2 The best extraction process of gardenia yellow pigment from Fructus Gardeniae are ethanol concentration of 50%, extraction temperature of 60 ℃, extracting time of2 hours, solid-liquid ratio of 16 m L/g, and extract twice by orthogonal experimental design method.3 In the experiments of lowering postprandial blood sugar in mice, we know thewater-soluble alkaloids fraction from Cortex Lycii can stabilize the postprandial blood glucose of mice. The effect of stabilizing postprandial blood sugar has relatively similar results with acarbose.Conclusion1 It is feasible to optimize the extraction process of traditional Chinese medicine effective parts by orthogonal experiment design and response surface design methods2 The water-soluble alkaloids from Cortex Lycii can stabilize postprandial blood sugar in mice.