Research About Antagonism Induced By Sevoflurane And Insulin Postconditioning Against Myocardial Ischemia-reperfusion Injury

Objective:To study the cardioprotection of sevoflurane-postconditioning and GIK-postconditioning in type 1 diabetes mellitus rat hearts.Method: 61 SD rats were randomly devided into 6 groups: Normal, Control, DR, DSR, DIR, DISR. T1 DM models were induced by STZ injection. Myocardial ischemia-reperfusion models were established by LSD occusion. Sevoflurane-postconditioning was done by inhalation of 2% sevoflurane. GIK-postconditioning was done by vascular injection of GIK. Heart tissues were dyed by TTC and slice into pieces and infarct size was analyzed by Image J. Plasma c Tn I and CK-MB measurements were done by chemiluminescence analysis. Akt activation was detected by Western Blotting.Results: Compared to Control group, infarct size was significantly larger in DR and DISR(P<0.01)while it is smaller in DIR, plasma c Tn I levels in Normal were lower(P<0.05) while extremely higher in the other 4 groups(P<0.01, P<0.01, P<0.01, P<0.01), plasma CK-MB levels were lower in DR(P<0.05), cardiac p-Akt expression was markedly decreased in Normal and DISR(P<0.01, P<0.01) while distinctly increased in DR(P<0.01). Compared to DR group, infarct size was smaller in DISR(P<0.05) while obviously smaller in DSR and DIR(P<0.01, P<0.01), plasma CK-MB levels were extremely lower in DIR(P<0.01), cardiac p-Aktexpression was signally decreased in DSR, DIR and DISR(P<0.01, P<0.01, P<0.01).Conclusions: Sevoflurane-postconditioning provides protective effect in T1 DM rats undergone myocardial ischemia-reperfusion. This effect is weakened by GIK-postconditioning.