| Purpose: To explore the effect and the related mechanism on the apoptosis of HepG2 cell induced by rosmarinic acid and the related mechanism. Methods: 1. MTT method was used to determine the inhibition effects on the growth of HepG2 cells after rosmarinic acid treat for 24 hhours, 48 hours and 72 hours, and the cell shapes change was observed with the microscope. 2. Flow cytometry was used to test the cell apoptosis. 3. Flow cytometry was used to determine the change caused by rosmarinic acid. 4. Werstern Blotting was used to check the P53, c-Myc, PARP protein expression of HepG2 after rosmarinic acid treatment. Results: 1. HepG2 cell growth was inhibited by 6.25,12.5,25 and 50μg/ml rosmarinic acid for 24 and 48 h. Meanwhile, the cell shape was changed to shrinking and aggregation. IC50 is 46.973±1.709 μg/ml. 2. HepG2 cell growth was inhibited by 6.25,12.5,25 and 50μg/ml rosmarinic acid for 24 and 48 h. Meanwhile, rosmarinic acid had the effect on early apoptosis of HepG2 cells. 3. HepG2 cell growth was inhibited by 6.25,12.5,25 and 50μg/ml rosmarinic acid for 24 and 48 h. Meanwhile, the cell proportion increased in G0/G1 period compared with the control group. 4. HepG2 cell growth was inhibited by 6.25,12.5,25 and 50μg/ml rosmarinic acid for 24 and 48 h. Meanwhile, the expression of PARP, c-Myc decreases with the concentration increasing of the acid, and the expression of P53 increases with the concentration increasing of the acid. Conclusion: 1. Rosmarinic acid can inhibit the growth of HepG2 cells. 2. Rosmarinic acid blocks HepG2 in G0/G1 period. 3. Rosmarinci acid can activate anti-oncogene p53, and accelerate the apoptosis of HepG2. |
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