Effects Of Over-expressed S100A6 On Biological Features Of Eutopuic Endometrial Stromal Cells And Its Interact With β-catenin

Objectives: To investigate the influence of over-expressed S100A6 on proliferation,migration and apoptosis in eutopic endometrial stromal cells and its interaction with β-catenin.Methods: The eutopic endometrial stromal cells were cultivated in vitro and divided into Lv-S100A6 group and negative group, which infected with the recombinant lentivirus S100A6(Lv-S100A6) and recombinant lentivirus vector respectively. CCK-8 assay was performed to identify the proliferation of cell. The cell migration was detected by Transwell chamber. Flow cytometry method was performed to detect cell apoptosis. Western blotting and RT-PCR were performed to identify the expression of β-catenin gene.Results: As compared with the blank group and negative control group, the proliferation was increased in eutopic endometrial stromal cells after transfection with Lv-S100A6(P<0.05), the ability of migration was promoted. However, the apoptosis rate in eutopic endometrial stromal cells after transfection with Lv-S100A6(2.99%)was decreased compared with the blank control group(13.48%) and the negative group(14.40%)(P<0.05). The Western blot analysis showed that β-catenin expression was significantly higher in S100A6-transfected cells compared with vector control, following normalization to β-actin levels(0.314±0.012, 0.326±0.008, 0.668±0.016 for blank, negative, and Lv-h S100A6 transfected cells, respectively, p<0.05). In addition, the PCR analysis also showed that β-catenin m RNA levels were highest in Lv-S100A6-transfected cells, whereas no significant difference was detected between negative group and blank group cells(0.498±0.012, 0.512±0.011, 0.837±0.010 in untreated, Lv-control, and Lv-S100A6-transfected cells, respectively, p<0.05). We also observed high expression of S100A6 m RNA in cells transfected with therecombinant S100A6 lentivirus construct(Lv-h S100A6), compared with blank and vector control cells(negative group), The relative ratio of S100A6 was 0.231±0.018, 0.242±0.021, and 0.495±0.015 in untreated, Lv-control, and Lv- S100A6 transfected cells).Conclusion: S100A6 can effectively increase the proliferation and migration in eutopic endometrial stromal cells, and inhibit the cell apoptosis, in addition, it canupregulation the expression of β-catenin.