Effects Of Lanthanum Chloride On Wear Particle-induced Osteolysis In A Mouse Calvarial Model In Vivo

Objectives:Purpose The aim of this study was to determine the effects of lanthanum chloride(La Cl3) on osteolysis in vivo.Methods:Research was performed on 32 male C57BL/6J mice that were divided into four groups with eight mice in each group: sham group with saline, vehicle group, wear particles +(1 mg kg-1 d-1)low dosage La Cl3 group and wear particles +(5 mg kg-1 d-1)high dosage La Cl3 group. Using Elisa method to detect content of serum TNF- α, IL-1 β, CTX-I. Micro-computed tomography, hematoxylin-eosin staining and tartrate-resistant acid phosphatase staining were applied for the pathological investigation of calvaria.Results:In mice treated with high-dose La Cl3, particle-induced osteolysis and inflammatory reaction were reduced compared with that in the vehicle-treated control. Moreover, treatment with high-dose La Cl3 suppressed the wear particle-induced decrease in bone mineral content, bone mineral density, and bone volume fraction. Bone destruction and resorption were higher in the La Cl3-treated group than in the saline-treated group but lower than that in the wear particle group. The result indicated that La Cl3 treatment could inhibited wear particle-induced inflammatory reaction and osteolysis in vivo.Conclusion:La Cl3 can effectively inhibit wear partiles-induced inflammatory reaction and osteolysis through the inhibition of osteoclastogenesis and might be used in the treatment or prevention of wear particles-induced osteolysis and aseptic loosening.