Preclinical Pharmacological Study On Mongolia Bone Setting Medicine Pill

This paper mainly studied preclinical studies of Mongolia Bone Setting Medicine Pill,through Mongolia Bone Setting Medicine Pills’ pharmacodynamic experimental study, Pharmacology and experimental study of drug safety, these experiments provide the basis for further development and utilization.In a pilot experiment, LD50 can not be measured, therefore measure the maximum tolerated dose. In formal tests, the mice received orally by gavage the maximum tolerated oral dose of Mongolia Bone Setting Medicine Pill, and weight and status and survival for 14 days post administration were observed. Experimental results show that mice without death, in good condition, the organ has not changed. The results indicated that Mongolia Bone Setting Medicine Pill is not toxic.To investigate the anti-inflammatory effects of Mongolia Bone Setting Medicine Pill on mice. The anti-inflammatory effects were tested by dimethylbenzene-induced ear swollen method and egg-induced paw edema in mice. To determine the test mouse ear swelling rate and tissue swelling rate. The results indicated that Mongolia Bone Setting Medicine Pill had marked inhibitory effects on inflammation models induced by Dimethylbenzene and eggwhite.To investigate the analgesic effects of Mongolia Bone Setting Medicine Pill on mice. The analgesic effects were tested by hot plate method and body twisting method in mice. To determine the number of body writhing and the time of the mice first licking. The results indicated that Mongolia Bone Setting Medicine Pill had marked inhibitory effects on paid models induced by wring body and hot board methods in mice.To investigate the anti-fatigue effect of Mongolia Bone Setting Medicine Pill on Mice. Record mice swimming time and measure serum substances after mice swimming without weight. The results indicated that, Mongolia Bone Setting Medicine Pill can significantly prolong the mice swimming exhaustive time,significantly increased LDH activity in mice, LA levels and BUN levels in mice significantly reduced. Mongolia Bone Setting Medicine Pill possess relieving physical fatigue effect.The study was to identify the influence of Mongolia Bone Setting Medicine Pill solution on fracture union by experimental study and clinical preliminary observation. By radial fractures in rats, observed X-rays and tested in rat serum calcium concentration, phosphorus concentration and alkaline phosphatase concentrations, and biomechanical measurement, observe the effect of Mongolian medicine pill treatment of bone fractures. The results indicated that Mongolia Bone Setting Medicine Pill can accelerate bone healing, bone loading dose Mongolia Bone Setting Medicine Pill significantly better than the control group. Mongolia Bone Setting Medicine Pill has a good role in the treatment of fractures.